慢性吗啡处理对大鼠恐惧消退损伤效应研究

发布时间：2018-03-23 06:21

本文选题：慢性吗啡处理　切入点：恐惧消退　出处：《南华大学》2007年硕士论文　论文类型：学位论文

【摘要】： 目的:恐惧消退异常或缺陷常导致焦虑障碍等疾病发生;另一方面,流行病学调查表明阿片滥用人群焦虑障碍等情感性疾病的发生率明显高于正常人群,其机制仍不清楚。本研究拟检测慢性吗啡处理是否损伤大鼠条件化恐惧消退过程,以期探讨阿片滥用伴焦虑障碍的可能神经机制。方法:连续10天吗啡处理(sc,递增剂量模式,每天2次),建立阿片依赖动物模型。戒断后28小时与第7天,行自发戒断症状检测。戒断后第7天,行线索性(声刺激与足底电击配对)或场景性(特定场景与足底电击配对)恐惧条件化训练;第8天至第11天,行线索性(声刺激单独呈现)或场景性(场景单独呈现)恐惧消退训练(每天1次);行为检测指标为声刺激或场景诱发的僵直时间。非特异性反应的检测包括:痛域(热水浴甩尾法、活动度)、声刺激非特异性僵直与场景非特异性僵直。结果:1、戒断后28小时,吗啡组大鼠与生理盐水组大鼠相比表现出明显的戒断症状;戒断后第7天,戒断症状不明显。2、在线索性恐惧条件化阶段,吗啡组僵直时间与生理盐水组相比无显著性差异;在线索性恐惧消退阶段,吗啡组僵直时间显著性高于生理盐水组,但每次消退训练期间的消退比率无显著性组间差异。3、在场景性恐惧条件化与恐惧消退阶段,吗啡组僵直时间与生理盐水组相比均无显著性差。4、非特异性反应检测均无显著性组间差异。结论:1、本实验吗啡处理程序能可靠地诱导大鼠对吗啡的依赖。2、慢性吗啡处理损伤长时程线索性恐惧消退记忆,而对短时程线索性恐惧消退记忆无影响,该损伤效应并非慢性吗啡处理导致非特异性反应改变而引起。3、慢性吗啡处理对场景性恐惧条件化与恐惧消退无影响。4、慢性吗啡处理所致恐惧消退缺陷可能介导了阿片滥用伴焦虑障碍等情感性疾病发生。
[Abstract]:Objective: abnormal or defective fear regression often leads to the occurrence of anxiety disorder. On the other hand, epidemiological investigation shows that the incidence of affective diseases such as anxiety disorder in opioid abusing population is significantly higher than that in normal population. In order to explore the possible neural mechanism of opioid abuse with anxiety disorder, the present study is to examine whether chronic morphine treatment can damage the process of conditioned fear regression in rats. Methods: the animal model of opioid dependence was established by morphine treatment for 10 consecutive days, with an increasing dose pattern, twice a day. The symptoms of spontaneous withdrawal were detected at 28 hours and 7 days after withdrawal, and 7 days after withdrawal. Fear conditioning training (acoustic stimulation and plantar shock pairing) or scene (specific scenario and plantar shock pairing); from day 8 to day 11, Fear retreat training was performed for cues (acoustic stimuli alone) or scene (scene alone) (1 time per day); behavior testing was measured by the time of stiffness induced by acoustic stimulation or scene. Detection of nonspecific responses included:. Pain area (hot water bath tail flick method, Range of activity, acoustic stimulation of non-specific stiffness and scene non-specific stiffness. Results at 28 hours after withdrawal, the morphine group showed obvious withdrawal symptoms compared with the normal saline group, and on the 7th day after withdrawal, the withdrawal symptoms were not obvious. The stiffness time of morphine group was significantly higher than that of saline group, while that of morphine group was significantly higher than that of saline group. However, there was no significant difference in the regression ratio between groups during each receding training period, but in the phase of scene fear conditioning and fear regression, there was no significant difference between the two groups. There was no significant difference in stiffness time between morphine group and saline group, and there was no significant difference in nonspecific reaction between groups. ConclusionThe morphine treatment program can reliably induce morphine dependence in rats, chronic morphine treatment can induce long-term clue fear fading memory, but it has no effect on short-term clue fear regression memory. The damage effect was not caused by the non-specific response change induced by chronic morphine treatment. Chronic morphine treatment had no effect on situational fear conditionality and fear regression. The defect of fear regression induced by chronic morphine treatment may mediate. Opioid abuse with anxiety disorders and other affective disorders occur.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

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