人类巨细胞病毒感染对脐血粒单系祖细胞增殖过程中HOXA5，HOXB6基因表达的影响

发布时间：2018-03-24 12:12

本文选题：巨细胞病毒　切入点：粒单系祖细胞　出处：《泸州医学院》2006年硕士论文

【摘要】：目的:本课题主要探讨人类造血干祖细胞(Hematopoietic Stem-Progenitor Cell,HSPC)向粒单系(Colony Fortming Unit-Granulocyte-macrophage,CFU-GM)增殖过程中HOXB6,HOXA5基因表达的情况,并且用人类巨细胞病毒(Human Cytomegalovirus,HCMV)和/或全反式维甲酸(all-trans-retinoticacid,ATRA)进行干扰,以探讨HCMV、ATRA在此过程中对HOXB6、HOXA5基因的影响,以进一步明确HCMV抑制脐血粒单系祖细胞增殖的机制,以及ATRA能有效治疗粒系白血病,尤其是急性早幼粒细胞白血病的可能机制。方法:1.脐血标本由本院产房提供,取自正常足月顺产新生儿断脐后的胎盘段脐血。2.实验分组。实验分4组:(1)空白对照组:不加病毒液,代之等量IMDM加入基本培养体系。(2)HCMV组:HCMV—AD_(169)滴度为组织培养半数感染量(tissue culture infectious,TCID_(50))10~(-4.5)/0.1ml,按100TCID_(50) 0.1ml感染培养体系。(3)ATRA组:加入ATRA稀释液0.1ml,终浓度60nmol/(?)l。(4)HCMV+ATRA组:同时加入HCMV—AD_(169)病毒稀释液0.1ml及ATRA0.1ml,终浓度同上。3.采用造血祖细胞体外培养技术,以HCMV—AD_(169)和/或ATRA持续干扰人类造血干祖细胞,观察正常组、HCMV—AD_(169)组、ATRA组、以及ATRA+HCMV组的人类造血干祖细胞经人粒细胞巨噬细胞集落刺激因子(Granulocyte-macrophage colony-stimulating,GM-CSF)诱导
[Abstract]:Objective: to explore human hematopoietic stem progenitor cells. Colony Fortming of Stem-Progenitor Cell-HSPCs. The expression of HOXB6 / HOXA5 gene during the proliferation of Unit-Granulocyte-macrophaget-macrophage (CFU-GM) was investigated by using human cytomegalovirus (human cytomegalovirus) and / or all-trans retinoic acid all-trans-retinoic acid (ATRAA) to investigate the effect of HCMVATRA on HOXB6 HOXA5 gene. To further clarify the mechanism of HCMV inhibiting the proliferation of cord blood granulocyte progenitor cells and the possible mechanism that ATRA can effectively treat myeloid leukemia, especially acute promyelocytic leukemia. Umbilical cord blood of placental segment taken from normal full-term spontaneous delivery newborns after umbilical cord breakage. 2. The experiment was divided into 4 groups: control group (control group): no virus solution, Instead of adding the same amount of IMDM to the basic culture system. 1: HCMV-ADS169) titer of tissue culture Infectious culture Infectious culture 10 ~ (-4. 5)% -0.1 ml, according to 100 TCT + + 50) 0.1ml infection culture system .3ATRA group: add 0.1 ml ATRA diluent, final concentration 60 nmol /??? HCMV ATRA group: HCMV-ADS169) virus diluent 0.1ml and ATRA 0.1ml were added simultaneously, the final concentration was same as .3.The hematopoietic progenitor cell culture technique was used to continuously interfere with human hematopoietic stem progenitor cells by HCMV-ADS-169) and / or ATRA, and to observe the normal group HCMV-ADstruc-1699) group, and to observe the effect of HCMV-ADS1699) on human hematopoietic stem progenitor cells, and to observe the effect of HCMV-ADS1699 on human hematopoietic stem progenitor cells. Human hematopoietic stem progenitor cells in ATRA HCMV group were induced by granulocyte-macrophage colony-stimulating factor GM-CSF.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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