HCK与IFRD1基因在胚胎心脏中时空表达的研究

发布时间：2018-03-25 21:11

本文选题：HCK基因　切入点：大鼠　出处：《南京医科大学》2007年硕士论文

【摘要】： 【摘要】目的检测HCK(hemopoietic cell kinase)基因在大鼠胚胎心脏发育过程中mRNA表达的变化，分析其与心脏发育的关系。方法取大鼠胚胎12、15、19d心脏，采用RT-PCR技术检测心脏组织中HCK基因mRNA的表达丰度，并应用原位杂交技术对HCK基因表达进行定位分析。结果在胚胎12、15、19d心脏中，HCK基因均有表达，但主要表达于心脏间质，在心肌细胞中不表达，其表达丰度分别为0.19±0.09、0.38±0.07、0.59±0.10，随胚龄增加呈逐渐上调趋势(P＜0.05)。结论HCK基因表达于心脏间质，在心肌细胞中无表达，其表达丰度与大鼠胚胎心脏发育时间有关，可能参与心脏发育。目的检测大鼠胚胎心脏发育过程中IFRD1(interferon-related developmental regulator 1)基因mRNA与蛋白的表达变化。方法取大鼠胚胎12、15、19d心脏，应用RT-PCR、Western blot、免疫组化技术，检测IFRD1基因mRNA与蛋白的表达。结果在大鼠胚胎12、15、19d天心脏中，IFRD1基因mRNA与蛋白均有表达，且呈逐渐下调趋势；其蛋白主要表达于心房、心室、肌小梁等心肌组织中，而在心内膜垫、瓣膜、流出道、大血管内层等处均未见表达。结论IFRD1基因随大鼠胚胎心脏发育表达逐渐下调，特异表达于心房、心室、肌小梁等大鼠胚胎心脏组织中，可能参与心脏发育。
[Abstract]:[abstract]. Objective to detect the expression of HCK(hemopoietic cell kinase gene in rat embryonic heart and to analyze the relationship between mRNA expression and cardiac development. Methods the expression of HCK gene mRNA was detected by RT-PCR technique. In situ hybridization technique was used to localize the expression of HCK gene. Results the expression of HCK gene was found in the heart of 1215 ~ 19d embryo, but mainly in the interstitial of the heart, but not in the cardiomyocytes. The expression abundance of HCK was 0.19 卤0.09 卤0.38 卤0.070.59 卤0.10, which was gradually up-regulated with the increase of embryonic age (P < 0.05). Conclusion the expression of HCK gene is expressed in the interstitial of the heart, but not in the cardiomyocytes, and its expression abundance is related to the development time of rat embryonic heart and may participate in the development of the heart. Objective to detect the expression of IFRD1(interferon-related developmental regulator 1 (IFRD1(interferon-related developmental regulator 1) gene mRNA and protein during the development of rat embryonic heart. Results the expression of mRNA and protein of IFRD1 gene was down-regulated in the rat heart on day 1215 and 19. The protein was mainly expressed in myocardium such as atrium, ventricle and trabecula, but in endocardium. Conclusion the expression of IFRD1 gene is down-regulated gradually with the development of rat embryonic heart, and is specifically expressed in rat embryonic heart tissues such as atrium, ventricle and trabecula, which may be involved in cardiac development.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R321.5

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