肺炎克雷伯菌致SD大鼠重症肺炎模型的建立及相关实验研究

发布时间：2018-03-26 16:48

本文选题：大鼠模型　切入点：重症肺炎　出处：《第一军医大学》2006年硕士论文

【摘要】：目的：重症肺炎目前国内外尚无统一的定义，从临床表现看，重症肺炎患者有严重的呼吸窘迫症状，血液动力学不稳定，需要吸入高浓度的氧，严重者需要机械通气支持，补充液体和血液动力学支持。目前，针对肺炎、尤其是一些重症肺炎的治疗，使用抗菌素治疗效果并不理想。仍然表现为疗效差，死亡率高等特点。国内、外学者正在积极探索对重症肺炎新的治疗途径，如细胞因子、免疫治疗等。为此，迫切需要建立细菌性重症肺炎的动物模型，以探索细菌性重症肺炎的诊断标准及病理生理特点，为重症肺炎治疗新途径的实验性治疗和药效学研究提供平台。方法：清洁级Sprague Dawleg(SD)大鼠随机分为模型组、观察组和对照组，每组分为3小组，模型组和观察组每小组8只，对照组每小组4只，，共计60只。其中，模型组和观察组分别接种相同浓度不同剂量的肺炎克雷伯菌菌液，对照组接种剂量相同的生理盐水。分别于接种后的第2、4、6天分批处死动物。动态观察动物的血液动力学、血气分析、外周血象、肺泡灌洗液(BALF)中白细胞和中性粒细胞计数及病理改变。同时，动态观察动物的干扰素-γ(IFN-γ)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的变化情况。留取肺组织标本，HE染色，每张切片随机取3-4个视野，用Image-Pro图像分析软件分别测试肺实质红细胞体积密度、支气管残余管腔体积密度、肺泡体积密度。结果：1、接种高剂量菌液的模型组于接种后第5天出现明显血液动力学改变，于第4天出现血气分析的改变。模型组外周血象、肺泡灌洗液的
[Abstract]:Objective: there is no unified definition of severe pneumonia at home and abroad. From the clinical manifestation, severe pneumonia patients have serious respiratory distress symptoms, hemodynamic instability, need to inhaled high concentration of oxygen, severe patients need mechanical ventilation support. Supplementary fluid and hemodynamic support. At present, for the treatment of pneumonia, especially some severe pneumonia, the effect of using antibiotics is not ideal. Still, it is characterized by poor curative effect and high mortality rate. At home, Foreign scholars are actively exploring new approaches to the treatment of severe pneumonia, such as cytokines, immunotherapy, etc. Therefore, it is urgent to establish animal models of bacterial severe pneumonia. To explore the diagnostic criteria and pathophysiological characteristics of bacterial severe pneumonia, and to provide a platform for experimental treatment and pharmacodynamics study of new approaches to severe pneumonia. Methods: clean grade Sprague Dawley SD rats were randomly divided into three groups: model group, observation group and control group. Each group was divided into 3 groups, the model group and the observation group (n = 8) and the control group (n = 4) each group (n = 60). The model group and the observation group were inoculated with the same concentration and different doses of Klebsiella pneumoniae solution respectively. The control group was inoculated with the same dose of normal saline. The animals were killed in batches on the 2nd day, 4th day after inoculation. The hemodynamics, blood gas analysis and peripheral blood picture of the animals were observed dynamically. The changes of leukocyte and neutrophil count and pathological changes in BALF (alveolar lavage fluid) were observed. The changes of IFN- 纬, IL-6, TNF- 伪 and TNF- 伪 were observed. Three or four visual fields were randomly selected for each section. The volume density of red blood cells in lung parenchyma and the residual lumen of bronchus were measured by Image-Pro image analysis software. Results the volume density of alveoli in the model group inoculated with high dose of bacterial fluid showed significant hemodynamic changes on the 5th day after inoculation and the change of blood gas analysis on the 4th day. The peripheral blood of the model group and the alveolar lavage fluid of the model group showed significant changes on the fifth day after inoculation and the change of blood gas analysis on the 4th day.
【学位授予单位】：第一军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332;R563.1

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