HIF-1α在脂多糖诱导的急性炎症反应中的表达及其意义

发布时间：2018-03-27 16:43

本文选题：HIF-1α　切入点：VEGF　出处：《苏州大学》2006年硕士论文

【摘要】： 目的:从小鼠体内和体外两方面,探讨LPS诱导的急性炎症组织中和体外培养的巨噬细胞内HIF-1α表达的改变及其意义方法:将Balb/c小鼠分为正常对照组及炎症组,炎症组采用卡介苗(BCG)配合脂多糖(LPS)尾静脉注射法建立小鼠急性全身性炎症损伤模型。取出肝脏和肺脏进行常规石蜡切片、HE染色观察脏器的病理改变,免疫组织化学SP法观察HIF-1α及VEGF在组织中的表达改变。另取正常Balb/c小鼠,分离腹腔巨噬细胞分为正常对照组、LPS刺激组,刺激组给予LPS、对照组给予PBS作用10小时后,采用RT-PCR法半定量检测HIF-1α、VEGF基因mRNA的表达水平,应用细胞免疫化学染色法检测巨噬细胞HIF-1α蛋白的表达水平。结果:炎症组小鼠肝脏及肺组织HIF-1α、VEGF表达较正常对照组显著增加(p均0.01)。体外培养中,与正常对照组比较,LPS刺激的巨噬细胞HIF-1α基因表达无明显改变(P0.05),但HIF-1α蛋白表达显著增加且VEGF基因表达上调(p均0.01)。结论:由LPS引起小鼠全身急性炎性时,肝和肺组织表达的HIF-1α及VEGF蛋白水平升高;体外常氧条件下,LPS刺激的小鼠巨噬细胞可在转录后水平上调HIF-1α蛋白质的表达,并可上调靶基因VEGF的表达;提示常氧条件下,LPS可通过转录后水平上调HIF-1α蛋白的表达从而参与体内的急性炎症反应。
[Abstract]:Objective: to investigate the changes and significance of HIF-1 伪 expression in acute inflammatory tissue induced by LPS and macrophages cultured in vitro and in vivo in mice. Methods: Balb/c mice were divided into normal control group and inflammatory group. The acute systemic inflammatory injury model of mice was established by BCG combined with lipopolysaccharide (LPS) injection into tail vein. The liver and lung were taken out for routine paraffin section and HE staining to observe the pathological changes of viscera. Immunohistochemical SP method was used to observe the expression of HIF-1 伪 and VEGF in normal Balb/c mice. The peritoneal macrophages were isolated and divided into two groups: the normal control group was stimulated by PBS, and the control group was treated with PBS for 10 hours. The expression of HIF-1 伪 -VEGF gene mRNA was detected by semi-quantitative RT-PCR method, and the expression of HIF-1 伪 protein in macrophages was detected by cytoimmunochemical staining. Results: compared with the normal control group, the expression of HIF-1 伪 -VEGF in the liver and lung tissues of the inflammatory group increased significantly. The expression of HIF-1 伪 gene in LPS-stimulated macrophages was not significantly changed, but the expression of HIF-1 伪 protein was significantly increased and the expression of VEGF gene was up-regulated. Conclusion: the expression of HIF-1 伪 and VEGF protein in liver and lung tissues increased during acute inflammation induced by LPS, and the expression of HIF-1 伪 protein was up-regulated by LPS-stimulated mouse macrophages at posttranscriptional level. The expression of target gene VEGF could be upregulated, which suggested that lipopolysaccharide could up-regulate the expression of HIF-1 伪 protein at posttranscriptional level and participate in the acute inflammatory response in vivo.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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