阴虚热盛型2型糖尿病动物模型的初步研究

发布时间：2018-03-29 21:31

本文选题：糖尿病　切入点：动物模型　出处：《辽宁中医药大学》2006年硕士论文

【摘要】：目的：建立阴虚热盛型2．型糖尿病的病症结合动物模型，探讨阴虚热盛型2型糖尿病动物模型的成模标准。方法：将80只清洁级WISTAR大鼠随机分为正常对照组、高脂对照组、糖尿病空白对照组和阴虚热盛模型组4组。对模型组大鼠灌服中药干姜、附子，其余各组大鼠不做任何处理，连续4周，然后用玉女煎对模型组大鼠进行反证。结果： 1．中药造模后大鼠出现多饮、多食、多尿、身体消瘦、大便干燥、灌药时挣扎明显。舌暗红、少苔等征象。 2．客观指标：观察了血糖、血脂、皮质醇、胰岛素水平、甲状腺功能、血栓素B_2、6-Keto-PGF_(1a)水平：模型组血糖显著升高，与正常对照组比较有明显差异，与糖尿病空白对照组无明显差异。模型组血清甘油三酯、总胆固醇和低密度脂蛋白均较正常对照组高，有显著性差异。与糖尿病空白对照组比较无显著性差异。模型组血浆皮质醇水平明显高于正常对照组有显著性差异；与糖尿病空白对照组无明显差异。模型组胰岛素水平较正常对照组降低，与正常对照组有显著性差异；与糖尿病空白对照组无明显性差异。模型组大鼠FT_3升高，与各对照组有显著性差异。模型组大鼠和糖尿病空白对照组血栓素B2水平升高，于正常对照组有明显差异；模型组大鼠和糖尿病空白对照组6-Keto-PGF_(1a)水平降低，，与正常对照组有明显性差异；但模型组与糖尿病空白对照组无显著性差异。用玉女煎反证后以上指标有所恢复，其中甘油三酯、总胆固醇、血栓素B_2水平与反证前有显著性差异，接近糖尿病空白对照组。结论：在高脂加链脲佐菌素诱发糖尿病造模方法的基础上，再根据中医理论运用中药四气五味的药性来研制2型糖尿病的中医证候的动物模型是切实可行的。从而将阴虚热盛“证”动物模型与糖尿“病”动物模型结合起来，使糖尿病动物模型证候化，与中医临床辨证论治相一致，以便为广泛开展糖尿病的研究提供良好的动物模型。
[Abstract]:Objective: to establish an animal model of type 2 diabetes mellitus with deficiency of yin and heat. Methods: eighty clean grade WISTAR rats were randomly divided into 4 groups: normal control group, hyperlipidemia control group, diabetes blank control group and Yin deficiency heat Sheng model group. After 4 weeks, the rats in the model group were treated with Yusu decoction. Results:. 1. After making model of traditional Chinese medicine, the rats showed signs of polydrink, polyfood, polyuria, thin body, dry stool, obvious struggle when perfusing medicine, dark red tongue, less fur and so on. 2. Objective measures: blood glucose, blood lipids, cortisol, insulin level, thyroid function, thromboxane B _ (26-Keto-PGF) levels were observed:. The blood glucose in the model group was significantly higher than that in the normal control group, but there was no significant difference between the model group and the diabetic blank control group. Serum triglyceride, total cholesterol and low density lipoprotein in the model group were significantly higher than those in the normal control group, but there was no significant difference between the model group and the control group. The plasma cortisol level in the model group was significantly higher than that in the normal control group, but there was no significant difference between the model group and the diabetic blank control group. The insulin level in the model group was lower than that in the normal control group, and there was a significant difference between the model group and the normal control group, but there was no significant difference between the model group and the diabetic blank control group. FT_3 in the model group was significantly higher than that in the control group. The level of thromboxane B2 in the model group and the diabetic blank control group was significantly higher than that in the normal control group, and the level of 6-Keto-PGFS-1 a in the model group and the diabetic blank control group was significantly lower than that in the normal control group. However, there was no significant difference between the model group and the diabetic blank control group. The levels of triglyceride, total cholesterol and thromboxane B _ 2 were significantly different from those before reverse, which were close to the control group of diabetes mellitus. Conclusion:. Based on the method of diabetes induced by hyperlipidemia and streptozotocin, According to the theory of traditional Chinese medicine, it is feasible to develop the animal model of TCM syndromes of type 2 diabetes mellitus by using the medicine of four qi and five flavors of traditional Chinese medicine, so as to combine the animal model of "syndrome" of Yin deficiency and heat with the animal model of "disease" of sugar and urine. The syndromes of diabetic animal model are consistent with TCM clinical syndrome differentiation and treatment, so as to provide a good animal model for the extensive research of diabetes mellitus.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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