耐甲氧西林金黄色葡萄球菌环境影响因素研究

发布时间：2018-04-02 10:16

本文选题：耐甲氧西林金黄色葡萄球菌　切入点：环境因素　出处：《中华医院感染学杂志》2014年20期

【摘要】：目的揭示耐甲氧西林金黄色葡萄球菌(MRSA)产生的环境影响因素,为预防和控制MRSA的产生提供理论依据。方法用临床常用消毒剂对甲氧西林敏感金黄色葡萄球菌(MSSA)进行诱导,对比诱导前后的药敏结果并检测其耐药基因。结果 8株MSSA和实验室标准菌株ATCC 25923共9株菌中,经消毒剂诱导后有5株变为耐药,其中4株在临床常用消毒剂的持续加量作用下,其抑菌环内出现了有细菌生长或抑菌环直径变为零的现象,且均获得了mecA基因变成了MRSA,个别的还获得了smr基因,有的丢失了norA基因。结论 MSSA潜在耐药趋势,经小剂量临床常用消毒剂诱导可以发生基因突变而获得新的耐药基因变身为MRSA,提示消毒剂不合理使用是MRSA形成的重要原因之一,医疗机构急需强化消毒剂合理使用管理,以便遏止MRSA的产生,防止MRSA暴发流行。
[Abstract]:Objective to explore the environmental influence factors of methicillin-resistant staphylococcus aureus (MRSA) and to provide a theoretical basis for the prevention and control of MRSA.Methods Methicillin-sensitive Staphylococcus aureus was induced by common disinfectants in clinic. Results of the 8 strains of MSSA and the laboratory standard strain ATCC 25923, 5 strains became resistant after induced by disinfectant. Under the continuous dosage of disinfectants commonly used in clinical use, the growth of bacteria or the diameter of bacteriostasis ring turned to zero in the bacteriostasis ring of 4 strains, and the mecA gene was transformed into MRSA gene, and smr gene was obtained in some cases. Some of them lost the norA gene. Conclusion the trend of potential drug resistance of MSSA is that the gene mutation can be induced by small dose disinfectant and the new drug resistance gene can be transformed into MRSA, which indicates that the irrational use of disinfectant is one of the important reasons for the formation of MRSA. Medical institutions urgently need to strengthen the rational use and management of disinfectants in order to stop the production of MRSA and prevent the outbreak of MRSA.
【作者单位】：兰州军区兰州总医院感染控制科;中国科学院北京基因组研究所;兰州军区兰州总医院药剂科;
【基金】：甘肃省自然科学基金资助项目(3ZS051-A25-078)
【分类号】：R378.11

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