气道高反应动物模型的建立与指标检测

发布时间：2018-04-03 09:36

本文选题：气道高反应性　切入点：辣椒素　出处：《中国比较医学杂志》2014年02期

【摘要】：目的通过重新评价卵蛋白致小鼠哮喘模型,寻找一种简便易行的气道高反应动物模型和相应的检测指标,为研发治疗本类疾病药物和研究气道高反应发病机制提供新的实验手段。方法小鼠采用卵白蛋白(OVA)致敏;致敏后(15~21)d给予10%OVA雾化吸入激发哮喘,在末次激发24 h内测量小鼠辣椒素引咳的半数有效浓度,处死小鼠取肺组织测定匀浆中NO、IL-13、ET-1的含量。结果卵蛋白致敏模型小鼠随辣椒素浓度的升高其咳嗽反应阳性率、咳嗽次数明显增加(与对照组相比较P0.01)。模型组辣椒素引咳的半数有效浓度为89.39μmol/L,对照组、地塞米松组分别为204.84、220.02μmol/L;小鼠肺匀浆NO、ET-1、IL-13含量均明显增加,地塞米松可明显抑制NO的升高(与对照组相比较P0.01)。结论小鼠经卵蛋白致敏并连续激发7 d与临床气道高反应性(AHR)的多种特征相似,操作简便易行,稳定性高,故可作为气道高反应性的模型。辣椒素引咳阈值的测定、动物肺组织中NO、IL-13、ET-1含量的变化,可作为评价模型严重程度的指标。
[Abstract]:Objective to reevaluate the mouse asthma model induced by ovalbumin in order to find a simple animal model of airway hyperresponsiveness and the corresponding detection index.To provide new experimental means for the research and development of drugs for the treatment of this kind of disease and the pathogenesis of airway hyperresponsiveness.Methods the mice were sensitized with ovalbumin (ovalbumin), the asthma was stimulated by 10%OVA atomization inhalation on 1521 days after sensitization, the half effective concentration of capsaicin induced cough in mice was measured within 24 hours after the last stimulation, and the lung tissue of the mice were killed to determine the content of NO-IL-13 and ET-1 in the homogenate.Results with the increase of capsaicin concentration, the cough reaction positive rate and cough frequency of ovalbumin sensitized model mice were significantly increased (P 0.01) compared with the control group.The median effective concentration of capsaicin induced cough was 89.39 渭 mol / L in the model group, 204.84220.02 渭 mol / L in the control group and dexamethasone group, respectively.Conclusion Mouse ovalbumin sensitized and stimulated continuously for 7 days is similar to clinical airway hyperresponsiveness (AHR), which is easy to operate and stable, so it can be used as a model of airway hyperresponsiveness.The determination of capsaicin induced cough threshold and the change of NO-IL-13 ET-1 content in animal lung tissue can be used as an index to evaluate the severity of the model.
【作者单位】：北京中医药大学中药学院;
【基金】：北京中医药大学科研创新团队资助项目(编号:2011-CXTD-12)
【分类号】：R-332

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