诃子水提物对泛耐药鲍曼不动杆菌的抑菌作用

发布时间：2018-04-04 02:57

本文选题：诃子水提物　切入点：泛耐药鲍曼不动杆菌　出处：《中国微生态学杂志》2014年12期

【摘要】：目的研究诃子水提物对泛耐药鲍曼不动杆菌(PDR-Ab)的抑菌作用。方法采用二倍稀释法测定诃子水提物对临床分离的泛耐药鲍曼不动杆菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。小鼠腹腔注射上述PDR-Ab,建立急性肺炎模型。于造模前4天至造模后2天对小鼠连续灌胃给药,考察小鼠的死亡率、血液白细胞计数及肺组织病理变化。结果体外实验表明诃子水提物能抑制PDR-Ab在液体培养基中的生长,MIC和MBC分别为0.75 mg/m L和12 mg/m L。体内实验表明,剂量为150 mg/kg诃子水提物灌胃给药能明显降低PDR-Ab感染性急性肺炎小鼠的死亡率,使血液白细胞计数较快恢复,肺组织病理改善明显。结论诃子水提物对PDR-Ab在体内外均具有明显的抑菌作用,有望用于PDR-Ab感染性疾病的治疗。
[Abstract]:Objective to study the bacteriostatic effect of water extract of Fructus chebula on pan-resistant Acinetobacter baumannii PDR-Ab.Methods the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the aqueous extract of Fructus chebula against clinical isolates of Acinetobacter baumannii were determined by double dilution method.The model of acute pneumonia was established by intraperitoneal injection of PDR-Ab. in mice.From 4 days before the model to 2 days after the model, the mice were administrated by stomach continuously, and the mortality, blood white blood cell count and pathological changes of lung tissue were investigated.Results the in vitro experiments showed that the water extract of Fructus chebula could inhibit the growth of PDR-Ab in liquid medium. The MIC and MBC were 0.75 mg/m / L and 12 mg/m / L, respectively.In vivo experiments showed that the dosage of 150 mg/kg aqueous extract of Xanthoxopsis chebula could significantly reduce the death rate of mice with PDR-Ab infectious acute pneumonia, make the blood leukocyte count recover more quickly, and improve the lung pathology obviously.Conclusion the aqueous extract of Fructus chebula has obvious bacteriostatic effect on PDR-Ab in vivo and in vitro, and it is expected to be used in the treatment of PDR-Ab infectious diseases.
【作者单位】：大连医科大学药学院;.大连医科大学影像学院;大连市友谊医院呼吸内科;
【基金】：2013年辽宁省大学生创新训练项目甲类(201310161017) 辽宁自然科学基金(2013023037)
【分类号】：R378

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