大鼠下丘脑与孤束核间内吗啡肽阳性神经元的往返联系

发布时间：2018-04-04 18:13

本文选题：内吗啡肽　切入点：下丘脑　出处：《第四军医大学》2006年硕士论文

【摘要】：内吗啡肽(Endomorphin，EM)是与μ阿片受体的亲和力和选择性最高的内源性配体，可分为EM1和EM2两种亚型，具有明确的镇痛作用。EM阳性神经元的胞体在中枢内主要分布于下丘脑、孤束核等，在外周主要分布于感觉神经节，而EM阳性纤维及其终末的分布较为广泛，但主要存在于下丘脑、孤束核、脊髓和延髓背角等许多重要结构。以往的研究证明EM1阳性神经元、纤维及终末主要存在于上位中枢，EM2阳性神经元、纤维及终末则主要位于脊髓等下位中枢和感觉神经节，提示EM1可能主要在高位中枢发挥镇痛作用，而EM2主要参与对伤害性刺激初级传入的调控。下丘脑、孤束核是内脏感觉信息传递的重要通路，并参与痛信息的调控，这些核团之间是否有EM阳性纤维往返联系以及它们的EM阳性纤维及终末的来源如何目前仍未见报道。本研究利用荧光金(FG)逆行追踪与EM免疫荧光组织化学染色相结合的双标技术，，观察了孤束核和下丘脑之间EM能纤维的往返联系。将FG注入孤束核(以外侧亚核为中心)后，在下丘脑室旁核小细胞部、穹窿周区、下丘脑后区及弓状核等核团内都观察到数量较多的FG逆行标记神经元；EM1和EM2阳性神经元的胞体主要位于下丘脑背内侧核、腹内侧核
[Abstract]:Endomorphine is an endogenous ligand with the highest affinity and selectivity to 渭 opioid receptors. It can be divided into two subtypes: EM1 and EM2. The cell bodies of EM positive neurons are mainly distributed in the hypothalamus, nucleus solitarius and so on.EM-positive fibers and their terminals are widely distributed in the sensory ganglia, but mainly in the hypothalamus, nucleus tractus solitarius, spinal cord and dorsal horn of medulla oblongata and so on.Previous studies have shown that EM1 positive neurons, fibers and terminals are mainly located in the epigastric center of EM2 positive neurons, and the fibers and terminals are mainly located in the spinal cord and the sensory ganglion.The results suggest that EM1 may play an analgesic role in the high central nervous system, while EM2 may be involved in the regulation of nociceptive stimulation of primary afferent.Hypothalamus and nucleus solitarii are important pathways of visceral sensory information transmission, and participate in the regulation of pain information. Whether there is an EM-positive fiber connection between these nuclei, and how their EM positive fibers and their source of terminal are still unknown.In this study, the relationship between EM energy fibers in nucleus solitarius and hypothalamus was observed by using the double labeling technique of fluorescence FG retrograde tracing and EM immunofluorescence histochemical staining.After FG was injected into the nucleus of the solitary tract (with the lateral subnucleus as the center), the paraventricular nucleus of the hypothalamus, the perifornical area, was located in the paraventricular nucleus of the hypothalamus.In the posterior hypothalamus and arcuate nucleus, a large number of FG retrograde labeled neurons EM1 and EM2 positive neurons were observed in the dorsal medial hypothalamus and ventromedial nucleus.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R322

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