天津地区福氏志贺菌整合子携带及耐药性研究

发布时间：2018-04-08 22:19

本文选题：福氏志贺菌　切入点：耐药　出处：《中国病原生物学杂志》2014年02期

【摘要】：目的了解天津地区不同年份福氏志贺菌血清型分布,整合子携带情况,耐药性及其变化趋势。方法采用血清学方法对天津地区不同年份分离的56株福氏志贺菌进行分型;PCR扩增整合子整合酶及可变区,并测序;采用K-B法测定其对16种抗菌药物的敏感性。结果 1981~1983年分离株福氏志贺菌1类整合酶阳性率为87.50%(28/32),其中27株可变区含氨基糖苷类药物耐药基因aadA;2009~2011年分离株福氏志贺菌1类整合酶阳性率为91.67%(22/24),可变区及3'末端扩增均阴性。1981~1983年分离株福氏志贺菌2类整合酶均阴性;2009~2011年分离株福氏志贺菌2类整合酶阳性率79.17%(19/24),可变区含dfrA1、sat1、aadA1,介导对甲氧苄啶、链丝菌素和链霉素耐药。有17株福氏志贺菌1、2类整合酶均阳性。1981~1983年分离株福氏志贺菌对四环素、链霉素、氯霉素及甲氧苄啶/磺胺甲VA唑耐药率高,多重耐药率为65.63%;2009~2011年分离株福氏志贺菌对氨苄西林、链霉素、甲氧苄啶/磺胺甲VA唑、哌拉西林和四环素耐药率高,多重耐药率为83.33%。结论 1、2类整合子广泛存在于天津地区福氏志贺菌中,其中1类整合子3'保守末端可能缺失或存在变异。2009~2011年分离株福氏志贺菌对抗菌药物耐药性较1981~1983年分离株增强,多重耐药率增高。福氏志贺菌优势血清型发生转变,血清型分布呈现多样化。
[Abstract]:Objective to investigate the distribution of Shigella flexneri serotype, integron carrier, drug resistance and its changing trend in different years in Tianjin.Methods 56 strains of Shigella flexneri isolated from different years in Tianjin were genotyped by PCR to amplify integron integrase and variable region and sequenced, and the sensitivity to 16 antimicrobial agents was determined by K-B method.Results from 1981 to 1983, the positive rate of class 1 integrase of Shigella flexneri was 87.50 / 32, among which 27 strains of variable region containing aminoglycoside resistance gene aadAhor were 91.67% 22 / 24, variable region and 3'terminal extension were 91.67%, and 3'-terminal extension respectively, the positive rate of class 1 integrase of Shigella flexneri was 91.67%, and the positive rate of class 1 integrase of Shigella flexneri was 91.67% from 1989 to 2011.Streptomycin and streptomycin are resistant.17 Shigella flexneri strains were positive for class 1 and 2 integrases. Shigella flexneri isolated from 1981 to 1983 had high resistance to tetracycline, streptomycin, chloramphenicol and trimethoprim / sulfamethazol. The multidrug resistance rate of Shigella flexneri to ampicillin from 2009 to 2011 was 65.6363%.Streptomycin, trimethoprim / sulfamethazol, piperacillin and tetracycline showed high resistance, and the multidrug resistance rate was 83.33.Conclusion 1Class 2 integron is widely found in Shigella flexneri in Tianjin area. The 3'conservative terminal of class 1 integron may be absent or mutated. The antimicrobial resistance of Shigella flexneri isolated from 2009 to 2011 is stronger than that of the strain isolated from 1981 to 1983.The rate of multidrug resistance increased.The predominant serotype of Shigella flexneri changed and the distribution of serotype was diversified.
【作者单位】：天津医科大学第二医院天津市感染性疾病研究所;
【分类号】：R378.25

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