心房颗粒释放形式和转运途径的结构基础研究

发布时间：2018-04-09 05:11

本文选题：心房颗粒　切入点：心包膜　出处：《河北医科大学》2005年硕士论文

【摘要】：目的:放射免疫分析表明,心包腔的心包液中,存在心房尿钠多肽简称心房肽(ANP);而且,在生理或病理的情况下,心包液中心房肽的浓度,约为血浆中的4～100倍。这种浓度差提示,心包液中的心房肽,可能由心肌细胞直接分泌入心包腔。然而,关于心包腔中存在高浓度心房肽的生理和病理意义,日前仍不十分清楚。对于心包液中心房肽的来源和去向问题,尚缺乏形态学方面的证据。本研究通过对心房和心包的光镜和电镜观察,探讨心房颗粒(ASG)或心房肽的释放形式,研究心房颗粒或心房肽在细胞外的转运途径,为心包液中心房肽的来源、去向及其重要意义,提供结构基础和形态学方面的依据。方法:(1) 对家兔右心耳及其对应的心包膜,进行苏木精—伊红(HE)染色和5’核苷酸酶(5’-Nase)组织化学染色,应用光镜(LM),观察心外膜和心包膜的细微结构,以及其中的淋巴管分布特点。 (2) 应用透射电镜(TEM),观察右心耳和心包膜的超微结构,以及上皮细胞之间连接的特点,辨别心肌细胞之间毛细血管的类型;观察毛细淋巴管和心房颗粒的超微结构及其分布特征。 (3) 应用扫描电镜(SEM),观察心外膜表面和心包膜腔面的结构特点;对NaOH消蚀处理后的心包膜样品,观察间皮细胞下结缔组织纤维的构筑特征。
[Abstract]:Objective: radioimmunoassay showed that atrial natriuretic peptide (ANPP) was present in pericardial fluid of pericardium, and that the concentration of atrial peptide in pericardial fluid was 100 times higher than that in plasma under physiological or pathological conditions.This concentration difference suggests that atrial peptides in pericardial fluid may be secreted directly by cardiomyocytes into the pericardial cavity.However, the physiological and pathological significance of the presence of high concentrations of atrial peptides in the pericardium remains unclear.There is no morphological evidence for the origin and whereabouts of atrial peptides in pericardial fluid.The aim of this study was to investigate the release form of atrial granule ASG or atrial peptide by means of light and electron microscope observation of atrium and pericardium, and to study the pathway of translocation of atrial granule or atrial peptide in pericardial fluid, which was the source of atrial peptide in pericardial fluid.The destination and its significance provide the basis of structure and morphology.Methods the right atrial appendage and its corresponding pericardial membrane were stained with hematoxylin eosin (HEH) and 5 'nucleotidase 5' -Nase (Nase) histochemical staining. The fine structure of epicardium and pericardium was observed by light microscope.And the characteristics of lymphatic distribution.The ultrastructure of right atrial appendage and pericardium and the characteristics of the connection between epithelial cells were observed to distinguish the types of capillaries between cardiac myocytes, and the ultrastructure and distribution of capillary lymphatics and atrial granules were observed by means of transmission electron microscope (TEM).The structural characteristics of epicardial surface and pericardial cavity surface were observed by scanning electron microscope (SEM), and the architectural characteristics of connective tissue fibers under mesothelial cells were observed for pericardial samples treated with NaOH.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R322

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