JWA基因参与K562细胞应答氯化高铁血红素和热应激作用的可能机制

发布时间：2018-04-09 19:23

本文选题：JWA　切入点：K562细胞　出处：《南京医科大学》2006年硕士论文

【摘要】：人类疾病往往是环境因素与遗传因素共同作用的结果，探讨基因与环境的相互作用机制与规律，对于防治环境因素造成的机体危害具有重要意义。 1998年，周建伟等利用差异显示高通量筛选技术从培养的原代人的气管和支气管上皮细胞中分离克隆了一个在生物进化上高度保守的环境应答基因-JWA，其编码蛋白是一种新的微管相关蛋白，并受多种分化诱导剂如氯化高铁血红素(hemin)等调控。该基因可能参与多种白血病细胞的定向分化和凋亡，并与细胞应答环境中的热应激的信号转导通路有关。为了进一步深入了解JWA基因与红白血病的关系，本课题用hemin、热应激及hemin与热应激联合处理K562细胞，观察JWA基因在这些细胞模型中表达的特点和规律，探讨JWA在细胞应答hemin和应激信号通路中的作用和分子机制，以期为发展新的物理疗法与化疗结合治疗红白血病提供依据。一、JWA对hemin处理K562细胞的应答：
[Abstract]:Human disease is often the result of the interaction of environmental factors and genetic factors. It is of great significance to explore the interaction mechanism and law of genes and environment for the prevention and treatment of environmental hazards.In 1998,Zhou Jianwei et al used differential display high-throughput screening technique to isolate and clone a highly conserved environmental response gene, -JWA-JWA-JWA-JWA-JWA-JWA-JWA-JWA-JWA-JWA-JWA-JWA-JWA, a highly conserved environmental response gene, was isolated from cultured primary human tracheobronchialMicrotubule-associated proteins,It is regulated by many differentiation inducers such as hemin.This gene may be involved in the directional differentiation and apoptosis of various leukemic cells and may be related to the signal transduction pathway of heat stress in the cellular response environment.In order to further understand the relationship between JWA gene and erythroleukemia, K562 cells were treated with hemin, heat stress, hemin and heat stress, and the characteristics and regularity of JWA gene expression in these cell models were observed.To explore the role and molecular mechanism of JWA in cellular response to hemin and stress signaling pathway in order to provide evidence for the development of new physiotherapy combined with chemotherapy in the treatment of erythroleukemia.The response of JWA to K562 cells treated with hemin:
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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