不同年龄兔关节软骨细胞生物力学特性研究

发布时间：2018-04-11 14:47

  本文选题：年龄 + 软骨细胞　； 参考：《山西医科大学》2007年硕士论文

【摘要】： 目的:对不同年龄的兔关节软骨细胞的黏弹性进行定量的检测,以从细胞水平阐明关节软骨的退变过程,了解生物力学因素在骨性关节炎发病机理中的作用。为软骨组织工程中相关力学因素的研究提供新思路和新方法,为软骨组织工程种子细胞时机的选择提供理论依据。 方法:15只雌性新西兰白兔,按照年龄分为3组:幼年组5只(约6周龄)、成年组5只(约7月龄)、老年组5只(约31月龄)。各组实验动物均用空气栓塞处死,取左侧膝关节标本做大体观察,同时进行石蜡切片HE染色观察关节软骨随年龄增长的组织学变化;利用透射电镜观察随年龄增长关节软骨细胞超微结构的变化;利用激光共聚焦扫描显微镜(laser confocal scanning microscopeLCSM)对软骨细胞直径进行检测;利用微管吸吮技术(micropipette aspiration)结合半无限体细胞力学模型(Half-space model)对右侧膝关节所分离的软骨细胞进行黏弹性的检测。 结果:不同年龄兔关节软骨的大体观察及HE染色观察都有明显的变化,即关节软骨随着年龄的增长将会发生退行性改变;透射电镜观察发现老年组软骨细胞的超微结构发生了明显的变化;幼年软骨细胞的直径为8.84±0.97μm、成年软骨细胞的直径为9.77±1.41μm、老年软骨细胞的直径为11.50±1.76μm,统计分析表明三者两两之间的差异有统计学意义(p＜0.001)。关节软骨细胞表现为典型的黏弹性固体蠕变特性,随着年龄的增长,其黏弹性参数(瞬时模量E_0、平衡模量E_∞、表观黏性μ)逐渐降低,幼年组E_0(中位数±四分位数间距)为0.68±0.15kaPa、E_∞为0.38±0.16kPa、μ为6.42±1.36 kPa·s;成年组E_0为0.63±0.10kPa、E_∞为0.33±0.06kPa、μ为5.84±1.35 kPa·s;老年组E_0为0.56±0.08kPa、E_∞为0.28±0.05kPa、μ为4.06+0.71kPa·s。统计学分析表明幼年组及中年组的黏弹性特性均与老年组的差异有统计学意义(p＜0.005),而幼年组与中年组的黏弹性特性比较无统计学意义(p＞0.1)。 结论:不同年龄的关节软骨细胞都表现出典型的黏弹性固体蠕变特征;不同年龄关节软骨细胞的直径有明显的差别;老年关节软骨细胞的黏弹性特性较幼年及成年软骨细胞明显下降。
[Abstract]:Aim: to quantitatively detect the viscoelasticity of articular chondrocytes in rabbits at different ages in order to elucidate the degeneration process of articular cartilage at cellular level and understand the role of biomechanical factors in the pathogenesis of osteoarthritis.It provides a new idea and method for the study of related mechanical factors in cartilage tissue engineering, and provides a theoretical basis for the timing of seed cells in cartilage tissue engineering.Methods Fifteen female New Zealand white rabbits were divided into three groups according to their age: 5 young rabbits (about 6 weeks old), 5 adult rabbits (about 7 months old) and 5 elderly rabbits (31 months old).All the experimental animals were killed by air embolization. The specimens of the left knee joint were taken for gross observation, and the histological changes of articular cartilage with age were observed by HE staining in paraffin sections.The ultrastructural changes of articular chondrocytes were observed with transmission electron microscope (TEM), and the diameter of chondrocytes was detected by laser confocal scanning microscopeLCSM (laser confocal scanning microscope).Micropipette aspiration and Half-space model were used to detect the viscoelasticity of chondrocytes isolated from the right knee joint.Results: the gross observation and HE staining of articular cartilage in rabbits of different ages showed obvious changes, that is, the articular cartilage would degenerate with the increase of age.The ultrastructure of chondrocytes in the aged group was obviously changed by transmission electron microscope.The diameter of juvenile chondrocytes was 8.84 卤0.97 渭 m, that of adult chondrocytes was 9.77 卤1.41 渭 m, and that of senile chondrocytes was 11.50 卤1.76 渭 m.Articular chondrocytes showed typical viscoelastic solid creep properties. With age, the viscoelastic parameters (instantaneous modulus E _ 0, equilibrium modulus E _ 鈭，

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