某院鲍曼不动杆菌流行克隆株的分子特征

发布时间：2018-04-11 19:41

  本文选题：鲍曼不动杆菌 + 脉冲场凝胶电泳　； 参考：《解放军医药杂志》2014年02期

【摘要】：目的了解临床分离鲍曼不动杆菌的主要流行克隆株的变化趋势,揭示其分子流行的主要特征。方法筛选解放军253医院2010年6月—2012年12月临床分离的74株不重复鲍曼不动杆菌作为实验菌株,应用基于序列分型的多重聚合酶链反应(PCR)方法鉴定鲍曼不动杆菌的主要流行克隆谱系;利用限制性内切酶ApaI对实验菌株基因组DNA进行酶切和脉冲场凝胶电泳(PFGE)分析,并利用BioNumerics软件进行聚类分析;应用PCR及测序方法检测10种耐药相关基因(blaOXA-51-like、blaOXA-23-like、blaOXA-24-like、blaOXA-58-like、blaTEM、blaampC、armA、ISAba1、intI 1和intI 2)的携带情况。结果本组实验菌株克隆谱系以Group 1序列型为主,所占比例高达77.0%;PFGE带型表现出多样性,可大致分为13种图谱,其中主要型别为A型(35株);耐药相关基因blaOXA-51-like、blaOXA-23-like、blaTEM、blaampC、armA、ISAba1和intI 1的阳性率分别为100.0%、54.1%、86.5%、66.2%、89.2%、90.5%和85.1%,未检出blaOXA-24-like、blaOXA-58-like和intI 2。结论该院临床分离的鲍曼不动杆菌存在主要流行克隆株,与欧洲克隆谱系Ⅱ具有高度的同源性;多重耐药相关基因的携带率较高;不同的序列型或PFGE型别中存在同一耐药基因交叉分布的现象,说明多种耐药相关基因在鲍曼不动杆菌中发生着水平转移。
[Abstract]:Objective to investigate the variation trend of the main prevalent clones of Acinetobacter baumannii and reveal the main characteristics of the molecular prevalence of Acinetobacter baumannii.Methods 74 clinical isolates of Acinetobacter baumannii isolated from 253 Hospital of PLA from June 2010 to December 2012 were selected as experimental strains.The main prevalent clone lineages of Acinetobacter baumannii were identified by multiplex polymerase chain reaction (PCR) based on sequence typing, and the genomic DNA was analyzed by restriction endonuclease (ApaI) and pulsed field gel electrophoresis (PFGE).BioNumerics software was used to cluster analysis, and PCR and sequencing methods were used to detect the carrying of 10 genes related to drug resistance, such as blaOXA-51-like blaOXA-23-like blaOXA-like blaOXA-58-like blaOXA-58-like bla-TEMampCarmCarmCarmA, ISAba1 (intI1) and intI _ (2).Results the clone lineage of the experimental strains was dominated by Group 1 sequence type, the proportion of which was as high as 77.0% and showed diversity, which could be divided into 13 kinds of maps.Conclusion the clinical isolates of Acinetobacter baumannii have a high homology with European Clone 鈪，

本文编号：1737398