染氟成纤维细胞和成骨细胞IGF-1及bFGF的表达及其意义

发布时间：2018-04-12 13:47

本文选题：氟化物 + 胰岛素样生长因子1(IGF-1)　；参考：《吉林大学》2007年硕士论文

【摘要】： 成纤维细胞(fibroblast,FB)属于诱导性骨祖细胞范畴,在一定诱导因素的作用下具有向成骨细胞(osteoblast,OB)转化并发挥成骨功能的特点。IGF-1、bFGF与骨生长代谢密切相关,是骨代谢调控网络中的重要细胞因子,二者在促进FB和OB分化和增殖等方面有着重要的作用。本文将通过观察氟化物对FB和OB中IGF-1、bFGF表达水平的影响,探讨氟化物对OB成骨功能的促进作用和对FB成骨功能的诱导增强效应,并讨论这些作用在慢性氟中毒所致骨周软组织骨化和钙化的发生机制中的重要意义。以小鼠成纤维细胞株和小鼠颅骨成骨样细胞为实验对象,应用ELISA、免疫组化等方法,检测染氟FB和OB中IGF-1、bFGF蛋白的表达。结果表明,氟化物对FB和OB中IGF-1、bFGF蛋白的表达增强作用,在时间段和所需氟剂量上存在差异,但是具有相同的变化趋势。RT-PCR检查结果表明染氟FB中的IGF-1和bFGF mRNA表达增强的趋势明显。而且,IGF-1和bFGF对染氟后表达改变的时间段上存在一定的差异,提示二者在促进FB成骨功能增强方面可能具有互补作用。在单层细胞培养的基础上,建立三维培养体系,研究在更接近体内环境条件下,染氟FB中IGF-1蛋白和mRNA的表达及bFGF mRNA的表达。结果表明在两种培养体系中,染氟FB在IGF-1和bFGF表达增强的程度上存在一定的差异,但变化趋势相似,为证明氟化物对FB成骨功能的促进增强效应提供了进一步的佐证。
[Abstract]:Fibroblast fibroblast (FB) belongs to the category of induced osteoblast progenitor cells. It has the characteristics of transforming to osteoblastus OBB and exerting osteogenic function. IGF-1bFGF is closely related to bone growth and metabolism.They play an important role in promoting the differentiation and proliferation of FB and OB.By observing the effect of fluoride on the expression of IGF-1bFGF in FB and OB, the effects of fluoride on the osteogenic function of OB and the induction and enhancement of osteogenic function of FB were studied.The significance of these roles in the pathogenesis of ossification and calcification of periosseous soft tissue caused by chronic fluorosis was discussed.The expression of IGF-1bFGF in fluorinated FB (FB) and OB (OB) was detected by ELISA-immunocytochemistry in mouse fibroblasts and osteoblast-like cells.The results showed that fluoride could enhance the expression of IGF-1bFGF protein in FB and OB, but had the same change trend. RT-PCR results showed that the expression of IGF-1 and bFGF mRNA in FB exposed to fluoride was significantly increased.Moreover, there were some differences between IGF-1 and bFGF on the expression of FB after fluoride exposure, suggesting that they might play a complementary role in enhancing the osteogenic function of FB.On the basis of monolayer cell culture, a three-dimensional culture system was established to study the expression of IGF-1 protein and mRNA and the expression of bFGF mRNA in fluorinated FB.The results showed that in the two culture systems, there were some differences in the enhancement of FB expression in IGF-1 and bFGF, but the change trend was similar, which provided further evidence for the enhancement effect of fluoride on the osteogenic function of FB.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

【引证文献】