加热致小鼠睾丸生精障碍相关蛋白质及其功能的研究

发布时间：2018-04-13 16:46

本文选题：蛋白质组学 + 小鼠睾丸　；参考：《南京医科大学》2006年博士论文

【摘要】：精子发生是一个连续的细胞分裂分化过程，包括精原细胞分裂增殖、精母细胞减数分裂和精子形成三个主要阶段。精子发生过程涉及染色体数目的减半、遗传重组、染色质重塑及浓缩、精子顶体和尾部的形成等复杂的变化过程，所有这些高度有序的过程均需要相关蛋白质进行精确地调节。分离和鉴定与精子发生相关的蛋白质并探讨其相关功能对于揭示男性生殖的分子调控机制及临床男性不育症的诊治具有重要的理论和实践意义。为了研究小鼠睾丸局部短暂加热导致小鼠生精障碍的分子机制，我们运用蛋白质组学研究常用的技术手段—双向凝胶电泳(two-dimensional electrophoresis，2-DE)和质谱(mass spectrometry，MS)分离和鉴定小鼠睾丸加热后发生改变的蛋白质。首先我们创建了含有小鼠睾丸双向凝胶电泳参考胶的符合联盟标准的数据库，在参考胶上有504个通过肽质量指纹谱鉴定的蛋白点，点击胶上的蛋白点可链接到对应的蛋白质条目，同时还可得到蛋白质的鉴定信息，并可链接到SWISS-PROT或NCBI蛋白质网络数据库，另外，数据库支持复杂的查询功能，该公共数据库为研究正常小鼠睾丸功能和小鼠睾丸病理提供了有价值的资源；其次，我们采用类似的方法分析了小鼠睾丸局部
[Abstract]:Spermatogenesis is a continuous process of cell division and differentiation, which consists of three main stages: spermatogonia division and proliferation, spermatocyte meiosis and spermatogenesis.Spermatogenesis involves complex processes such as halving the number of chromosomes, genetic recombination, chromatin remodeling and condensation, and the formation of acrosome and caudal sperm. All these highly ordered processes need to be precisely regulated by related proteins.The isolation and identification of proteins associated with spermatogenesis and their related functions are of great theoretical and practical significance in revealing the molecular regulatory mechanisms of male reproduction and the diagnosis and treatment of clinical male infertility.In order to study the molecular mechanism of mouse spermatogenic disorder caused by local transient heating of testis in mice,Two dimensional electrophoresis2-DEE and MS were used to isolate and identify the proteins changed after testicular heating in mice by means of proteomics, two dimensional electrophoretic electrophoresis (2-DE) and mass mass spectroscopy (MSM).First of all, we created a database containing mouse testis two-dimensional gel electrophoresis reference gel that meets the alliance standard. There are 504 protein spots identified by peptide mass fingerprinting on the reference gel.Clicking on the protein dot on the glue links to the corresponding protein entry, and you can also get the protein identification information and link to the SWISS-PROT or NCBI protein network database. In addition, the database supports complex query functions.The common database provides valuable resources for the study of testicular function and testicular pathology in normal mice. Secondly, we used a similar method to analyze the local testis of mice.
【学位授予单位】：南京医科大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R321

【相似文献】