脂肪干细胞修复辐射诱导的肠血管损伤实验室研究

发布时间：2018-04-15 03:04

本文选题：脂肪干细胞 + 修复　；参考：《现代诊断与治疗》2014年22期

【摘要】：目的观察脂肪干细胞修复辐射诱导的肠血管损伤实验室研究。方法取辐射相关肠血管损伤患者脂肪组织以酶消化法处理后获取ADSCs,免疫荧光标记并体外培养、扩增后,注射至肠血管内,观察60d后肠血管修复情况,并分析体外培养、诱导下免疫荧光、流式细胞仪检测,并予以RT-PCR检测。结果诱导后肠血管特异标记Calponin、MHC、α-SMA,细胞核为红色荧光,细肌丝阳性表达可见绿色荧光。脂肪干细胞诱导后Calponin、MHC、α-SMA阳性表达率分别为39.26±2.54%、45.42±5.12%、48.22±4.48%,均相比诱导前阶段有明显差异(P0.05)。脂肪干细胞Calponin、MHC、α-SMA、SM-22αm RNA诱导后均发现相应m RNA表达。脂肪干细胞诱导情况下受损肠血管修复70%占比为100%,明显优于无诱导组(P0.05)。结论脂肪干细胞适当培养、诱导下可促进血管平滑及修复,利于消除血管损伤。
[Abstract]:Objective to observe the radiation-induced intestinal vascular injury induced by adipose stem cells in laboratory.Methods ADSCs were obtained from adipose tissue of patients with radiation-associated intestinal vascular injury treated by enzyme digestion. After amplification, ADSCs were amplified and injected into intestinal vessels. The repair of intestinal blood vessels was observed after 60 days, and the culture in vitro was analyzed.Induced immunofluorescence, flow cytometry, and RT-PCR detection.Results after induction, the intestinal vascular specific labeling of Calponinus MHC, 伪 -SMA.The nucleus was red fluorescence, and the positive expression of fine myofilament was green fluorescence.The positive expression rates of MHC and 伪 -SMA in adipose stem cells were 39.26 卤2.54 and 45.42 卤5.12, respectively.The expression of m RNA was found in adipose stem cells induced by MHC and 伪 -SMA-SM-22 伪 m RNA.The percentage of damaged intestinal vascular repair in adipose stem cells was 70%, which was significantly better than that in no induction group (P 0.05).Conclusion proper culture of adipose stem cells can promote smooth and repair of blood vessels and eliminate vascular injury.
【作者单位】：武汉大学基础医院解剖教研室;咸宁市中心医院同济咸宁医院;
【基金】：湖北省自然科学基金(301132174)
【分类号】：R329.2

【参考文献】