食管癌小鼠异位移植模型建立的初步探讨

发布时间：2018-04-15 09:06

本文选题：食管癌 + 动物模型　；参考：《湖南农业大学》2007年硕士论文

【摘要】： 目的建立人食管癌免疫SCID小鼠异位移植模型并对其转移性淋巴来源细胞特性进行初级研究。方法将食管癌细胞系Eca-109细胞悬液注射至SCID小鼠胃壁。三个月后或动物频死时处死解剖,观察肿瘤在小鼠体内的生长转移情况,将肉眼可见的纵膈淋巴结转移瘤块接种于SCID鼠皮下扩增,然后取小鼠皮下瘤组织进行细胞培养,得到稳定的细胞株后用MTT法分析细胞的生长曲线,Western bloting法检测拓扑异构酶的表达,酶谱法检测MMP-2,MMP-9的活性,划痕实验和Transwell体外移动实验检测细胞的移动能力。结果成功建立裸鼠胃壁筛选第一代高转移潜力细胞,并命名为NMC109,对其生物学特征研究发现其细胞增殖能力,移动能力和侵袭潜力都发生明显增强。结论:肿瘤胃壁移植是建立食管癌免疫缺陷动物自发性移植模型和筛选转移性细胞亚系的可行方法。
[Abstract]:Objective to establish the heterotopic transplantation model of human esophageal carcinoma immunized SCID mice and to study the characteristics of metastatic lymphoid cells.Methods the suspension of esophageal carcinoma cell line Eca-109 was injected into the gastric wall of SCID mice.After three months or when the animal died frequently, the tumor was killed and the tumor growth and metastasis were observed in mice. The mediastinal lymph node metastases were inoculated into SCID mice subcutaneously, and then the subcutaneous tumor tissues were taken from the mice for cell culture.After the stable cell line was obtained, the expression of topoisomerase was detected by Western bloting, the activity of MMP-2 and MMP-9 was detected by MTT assay, and the mobility of cells was detected by scratch test and Transwell in vitro mobility assay.Results the first generation of high metastatic potential cells were successfully established in nude mice gastric wall and named NMC109. The biological characteristics of NMC109 showed that the cell proliferation, mobility and invasion potential of NMC109 cells were significantly enhanced.Conclusion: tumor gastric wall transplantation is a feasible method for the establishment of spontaneous transplantation model and screening of metastatic cell sublines in immunodeficient esophageal carcinoma.
【学位授予单位】：湖南农业大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332;R735.1

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