储藏食物孳生粉螨研究
发布时间:2018-04-17 14:14
本文选题:粉螨 + 储藏食物 ; 参考:《安徽理工大学》2006年硕士论文
【摘要】:目的研究储藏食物孳生粉螨的种类和群落多样性,并调查肠螨病在从事食物加工储藏等人群中的流行情况及病原螨的种类。方法用电热集螨法和水膜镜检法对采集样本进行粉螨分离、鉴定和计数,并通过Margalef指数、Shannon-Wiener指数和Pielou指数,计算其物种丰富度、物种多样性和物种均匀度。通过询问病史、粪检以及血清总IgE、螨特异性IgE检测调查特殊人群中肠螨病的患病情况。结果1440份样本中,45种,695份样本有粉螨孳生,孳生密度为5.553±2.358只/克,其中储藏干果20种、储藏调味品10种、储藏水产品10种、储藏烘焙食品5种,总阳性率为48.3%(695/1440),而储藏干果为55.4%(306/552),储藏调味品为43.9%(158/360),储藏水产品为35.8%(129/360),储藏烘焙食品为60.7%(102/168)。分离粉螨23种,隶属于7科18属。物种丰富度指数在0.383~2.476之间,其中八月份最高;物种多样性指数在0.926~3.075,其中八月份最高;物种均匀度指数在0.752~0.981,,其中八九月份最高。不同类型储藏食物孳生粉螨群落参数不同,储藏干果孳生粉螨的物种丰富度指数(Margalef指数=2.148)和多样性指数(H'=2.995)最高;储藏调味品孳生粉螨的均匀度指数(J=0.978)最高。对289人作粪检,粪螨阳性18例,阳性率6.2%(18/289);其中单纯粪螨阳性3例。检出粉螨经鉴定有6种,即粗脚粉螨、腐食酪螨、粉尘螨、家甜食螨、隆头食甜螨、甜果螨。粪螨阳性者18例血清总IgE、螨特异性IgE分别为(86±24)IU/mL和(69±19)IU/mL,与粪螨阴性者相比,差异均具显著性(~ t=7.569,P<0.01;~☆t=8.009,P<0.01)。结论储藏食物粉螨污染严重,其中以6月到9月最为严重,需加强此阶段的粉螨防制。对从事食物加工、储藏的职业的人群应加强预防本病的知识教育,并要进行必要的劳动保护。
[Abstract]:Objective to study the species and community diversity of acaroid mites in food storage, and to investigate the prevalence of intestinal acariasis in the population engaged in food processing and storage and the species of pathogenic mites.Methods the species richness, species diversity and species evenness were calculated by Margalef index Shannon-Wiener index and Pielou index.The incidence of mesenteric acariasis in special population was investigated by asking history, fecal examination, serum total IgE and mite-specific IgE.Results of the 1440 samples, 45 species were acaroid mites with a density of 5.553 卤2.358 / g, including 20 dry fruits, 10 seasonings, 10 aquatic products and 5 baked foods, among which 20 were stored in dry fruit, 10 in seasoning, 10 in aquatic products, and 5 in baked goods, among which 20 were stored in dry fruits, 10 in seasonings, 10 in aquatic products and 5 in baked goods.The total positive rate was 48.3% 695% 1440%, while the storage of dried fruit was 55.4%, the storage of dried fruit was 55.6% 552%, the storage of seasoning was 43.9% / 360m, the storage of aquatic product was 35.8% 129R / 360m, the storage of baked goods was 60.710 2 / 168C.23 species of acaroid mites, belonging to 18 genera and 7 families, were isolated.The species richness index ranged from 0.383 to 2.476, with the highest in August, the highest in August, and the highest in August, and the highest in August.The species richness index (Margalef index = 2.148) and diversity index (H = 2.995) of acaroid mites in stored dry fruits were the highest, and the evenness index (J ~ (0.978)) of acaroid mites in storage condiments was the highest.In 289 cases, 18 cases were positive in feces mite, the positive rate was 6.2% and 289%, of which 3 cases were positive in pure feces mite.There are 6 species of acaroid mites identified, I. e., acaroid mites, Tyrophylopsis vulgaris mite, Dermatophagoides farinae, domestic sweet mite, rosehead sweet mite, sweet fruit mite.The specific IgE of feces mites in 18 cases were 86 卤24)IU/mL and 69 卤19 渭 mol / mL, respectively, which were significantly different from those of fecal mite negative patients.T0. 569 (P < 0. 01) ~ 8. 009 (P < 0. 01).Conclusion the contamination of acaroid mites in stored food is serious, especially from June to September. It is necessary to strengthen the prevention and control of acaroid mites in this stage.The population engaged in food processing and storage should strengthen the knowledge education on prevention of the disease and carry out necessary labor protection.
【学位授予单位】:安徽理工大学
【学位级别】:硕士
【学位授予年份】:2006
【分类号】:R384.4
【共引文献】
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