发育中小鼠胃肠道、胰腺中CCK-IR细胞和PP-IR细胞的形态学研究

发布时间：2018-04-18 14:10

本文选题：小鼠胃肠道 + 小鼠胰腺　；参考：《山西医科大学》2007年硕士论文

【摘要】： 目的:观察发育中小鼠胃肠道和胰腺内胆囊收缩素免疫反应细胞(cholecystokininimmunoreactive cells,CCK-IR细胞)和胰多肽免疫反应细胞(pancreatic polypeplideimmunoreactive cells,PP-IR细胞)的发生、分布、形态和数量变化。方法:应用免疫组织化学SP法观察小鼠胚胎第12天至出生后45天胃肠道和胰腺内CCK-IR细胞和PP-IR细胞的发生、分布及形态特征。结果:小鼠胚胎发育第16天小肠内出现CCK-IR细胞,第19天,结肠内出现CCK-IR细胞。小鼠的胃体部仅在胚胎第18、19天偶见CCK-IR细胞,生后未见CCK-IR细胞;而胃窦部始终未见CCK-IR阳性细胞。小鼠胰腺中的CCK-IR细胞,最早出现于胚胎第12天,出生后直至成年,胰腺中均可见到CCK-IR细胞。胚胎第16-19天小鼠的小肠绒毛上皮内可见散在的CCK-IR细胞;出生后,CCK-IR细胞多位于绒毛上皮和固有层的肠腺中。结肠的CCK-IR细胞分布在黏膜上皮和肠腺中。胰腺中的CCK-IR细胞主要分布在胰岛,染色深浅不一;外分泌部腺泡和导管的上皮细胞之间,可见散在、深染的CCK-IR细胞。统计结果表明,小肠:从胚胎期直至出生后发育成熟,CCK-IR细胞数量持续增多,出生后30天数量达高峰。其中,出生后1天、7天和15天组,回肠CCK-IR细胞的数量较十二指肠和空肠多。45天组,小肠的CCK-IR细胞数量显著减少。结肠:胚胎期偶见CCK-IR细胞。生后1-30天,CCK-IR细胞数量随发育增加,生后45天组,数量减少。胰腺:胚胎期CCK-IR细胞的数量以第15天最多,之后逐渐减少,生后胰腺内仅偶见CCK-IR细胞。小鼠胰腺中的PP-IR细胞最早见于胚胎第17天,结肠中的PP-IR细胞最早出现于胚胎第18天,出生后直至成年,胰腺和结肠中均可见PP-IR细胞。发育中小鼠的胃体、胃窦和小肠各段始终未见PP-IR细胞。发育中小鼠胰腺内PP-IR细胞主要分布于胰岛的周边部,外分泌部腺泡和导管的上皮内,可见散在的PP-IR细胞。统计结果表明,胰腺中PP-IR细胞从胚胎第17天至生后第15天,数量逐渐增多,并于第15天组数量达高峰。生后30天组数量减少,45天组数量再次增多。胚胎第18天到发育成熟小鼠结肠黏膜上皮和肠腺中始终可见PP-IR细胞,生后30天组,PP-IR细胞的数量较其它时段多。小鼠肠道和胰腺内CCK-IR细胞和PP-IR细胞的形态多样,可见免疫反应阳性细胞的突起伸至其它细胞之间。胰腺内的CCK-IR细胞有强弱两种阳性表达方式。结论:从胚胎第16天至出生后30天,小鼠小肠与结肠内的CCK-IR细胞数量持续增加,其分泌的CCK可能促进小肠和结肠的生长发育。发育中小鼠胰腺内CCK-IR细胞数量的变化提示,胰腺内表达的CCK其主要作用是刺激胰腺的生长、分化和发育。发育中小鼠胰腺和胰腺外器官中PP-IR细胞的分布,与人和其它种属动物间存在明显差异;胰腺和结肠中PP-IR细胞的数量变化提示,胰多肽可能参与调控胰腺和结肠的功能活动。 CCK-IR细胞和PP-IR细胞均可见免疫反应阳性细胞的突起伸至其它细胞之间,细胞外还可见免疫反应阳性物质逸出,这提示,两种内分泌细胞都可能以旁分泌方式影响周围细胞的功能活动。
[Abstract]:Objective: To observe the development of cholecystokinin immunoreactive cells in the gastrointestinal tract and pancreas in mice (cholecystokininimmunoreactive cells, CCK-IR cells) and pancreatic polypeptide immunoreactive cells (pancreatic polypeplideimmunoreactive cells, PP-IR cells) occurrence, distribution, morphology and number of changes.
Methods: immunohistochemical SP method was used to observe the occurrence, distribution and morphological characteristics of CCK-IR cells and PP-IR cells in the gastrointestinal tract and pancreas during twelfth days to 45 days after birth.
Results: the mouse embryos of CCK-IR cells in the small intestine, sixteenth days and nineteenth days, CCK-IR cells in the colon. The body of the stomach of mice at embryonic day 18,19 only a few CCK-IR cells, no CCK-IR cells after birth; and the gastric antrum had no CCK-IR positive cells in the mouse pancreas. CCK-IR cells first appeared on embryonic day twelfth after birth until adulthood, the pancreas can be seen in CCK-IR cells.
Embryonic day 16-19 mouse intestinal villi epithelium scattered within CCK-IR cells; after birth, CCK-IR cells located in the villous epithelium and lamina propria of the intestinal glands. Colon CCK-IR cells distributed in mucosa and intestinal gland. CCK-IR cells in the pancreas are mainly distributed in the islet, dyeing depth between the exocrine portion; acinar and ductal epithelial cells, scattered, deep staining of CCK-IR cells.
The statistical results showed that the small intestine: from the embryonic period until postnatal maturation, CCK-IR cell number continued to increase, 30 days after birth number reached the highest peak. Among them, 1 days after birth, 7 days and 15 days group, the number of ileum in CCK-IR cells compared with the duodenum and jejunum.45 day group, the number of CCK-IR cells was significantly reduced in small intestine I see. Colon: embryonic CCK-IR cells. 1-30 days after birth, the number of CCK-IR cells increased with the development, 45 days after birth. The decrease in the number of groups, the number of embryos: pancreatic CCK-IR cells for up to fifteenth days, then decreased gradually after birth, only occasionally seen in pancreatic CCK-IR cells.
The PP-IR cells in the pancreas of the mice were first seen on the seventeenth day of the embryo. The PP-IR cells in the colon first appeared on the eighteenth day of the embryo. After birth, they could see PP-IR cells in the pancreas and colon. There were no PP-IR cells in the gastric body, the antrum and the small intestine in developing mice.
The development of PP-IR cells and pancreas was mainly distributed in the periphery of the islets and exocrine acinar and ductal epithelial, scattered in PP-IR cells. The statistical results showed that the PP-IR cells in the pancreas from embryonic day seventeenth to fifteenth days after birth, the number gradually increased, reached the peak at fifteenth days after birth. The number of groups and 30 to reduce the number of days group, 45 days group increased again. The number of embryonic day eighteenth to always visible PP-IR cells mature mouse colon mucosa and intestinal gland in 30 days after birth, the number of PP-IR cells than the other periods.
The morphology of CCK-IR cells and PP-IR cells in the intestine and pancreas of mice is diverse. It is found that the protrusion of immunoreactive cells extends to other cells. There are two positive expressions of CCK-IR in pancreas.
Conclusion: from the 30 day embryo sixteenth days after birth, small intestine and colon of mice CCK-IR cells continued to increase, the secretion of CCK may promote the growth and development of small intestine and colon. Developmental changes in the number of CCK-IR cells in the pancreas and pancreas suggest that the expression of CCK and its main role is to stimulate pancreatic growth and differentiation development.
The distribution of PP-IR cells in the pancreas and extra pancreatic organs of developing rats is significantly different from that of humans and other species. The number of PP-IR cells in the pancreas and colon suggests that pancreatic polypeptide may participate in regulating the function of pancreas and colon.
Both CCK-IR cells and PP-IR cells showed the protrusion of immunoreactive cells extended to other cells, and the presence of immunoreactive substances also appeared outside the cell. This suggests that all two endocrine cells may influence the function of peripheral cells in paracrine manner.

【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R329

【参考文献】