外源性OT对成年小鼠下丘脑—垂体—性腺轴中GnRH表达及后代性别比例的影响

发布时间：2018-04-20 04:09

本文选题：催产素 + 促性腺激素释放激素　；参考：《西北农林科技大学》2007年硕士论文

【摘要】： 为了探讨催产素(OT)对促性腺激素释放激素(GnRH)的调节作用及其作用机制,本研究通过腹腔注射OT,采用免疫组化法观察OT对成年小鼠下丘脑-垂体-性腺轴中GnRH表达的影响;另外,为了研究OT对哺乳动物后代性比的调控作用,本研究通过腹腔注射和阴道注射OT观察OT对成年小鼠后代性别比例的影响。结果显示:给成年雄性小鼠腹腔注射OT,GnRH广泛分布于下丘脑-垂体-睾丸轴中,下丘脑GnRH的表达量与对照组显著降低,下降幅度与OT剂量呈剂-效关系。腺垂体GnRH的表达量与对照组相比都有降低,且OT低剂量组有极显著性差异;神经垂体中GnRH免疫阳性产物未见表达。结果表明OT对下丘脑-垂体系统GnRH表现抑制作用。GnRH免疫阳性产物主要分布于睾丸间质细胞和曲细精管的生精细胞和支持细胞,在被膜中未见阳性产物表达;睾丸间质GnRH的表达量与对照组相比都有升高,且OT低剂量组有极显著性差异;睾丸曲细精管GnRH的表达量随OT剂量的增加呈下降趋势,其中OT高剂量组与对照组相比有极显著性差异。结果表明OT对睾丸间质GnRH表现促进作用,而对曲细精管GnRH表现抑制作用。给发情前期成年雌性小鼠腹腔注射OT,GnRH广泛分布于下丘脑-垂体-卵巢轴中,下丘脑GnRH的表达量与对照组相比显著增加。腺垂体GnRH的表达量与对照组相比都有增加,且OT低剂量组有显著性差异;神经垂体中GnRH免疫阳性产物未见表达。结果表明OT对下丘脑-垂体系统GnRH表现促进作用。在卵巢的被膜、皮质和髓质未见阳性产物表达,而在黄体细胞和卵泡颗粒细胞中观察到有GnRH免疫阳性产物的存在;卵巢黄体和卵泡中GnRH表达量与对照组相比都有下降,其中OT低剂量组下降幅度大于OT高剂量组。结果表明OT对卵巢GnRH表现抑制作用。成年小鼠按雄给药组、雌给药组、雌雄给药组分别腹腔注射OT后,合笼交配,结果显示:雌给药组的不同剂量组的雌/雄性别比例都高于对照组,且高剂量组与对照组相比差异极显著。不同剂量组的雌/雄性别比例在雄给药组和雌雄给药组的变化趋势不定。结果提示外源性OT促进小鼠后代性别偏雌性,其影响依赖于给药剂量和给药方式。对成年雌性小鼠阴道注射OT,结果显示各剂量组的雌/雄性别比例都高于对照组,而且与剂量有正相关关系。结果表明子代小鼠雌/雄性比随OT剂量增加有上升趋势。提示OT作用于雌鼠有助于生偏雌性的后代。
[Abstract]:In order to investigate the effect and mechanism of oxytocin (OT) on gonadotropin releasing hormone (GnRH), the effect of OT on the expression of GnRH in the hypothalamus pituitary - gonadal axis of adult mice was observed by intraperitoneal injection of OT. In addition, in order to study the regulation effect of OT on the sex ratio of mammalian offspring, this study was conducted through this study. Intraperitoneal injection and vaginal injection of OT were used to observe the effect of OT on the sex ratio of offspring in adult mice.
The results showed that OT was injected into the abdominal cavity of adult male mice, and GnRH was widely distributed in the hypothalamus pituitary testicular axis. The expression of GnRH in the hypothalamus was significantly lower than that of the control group. The decrease was in the dose effect relationship with the dose of OT. The expression of GnRH in the adeno pituitary was lower than that of the control group, and there was a significant difference in the OT low dose group; G in the neurohypophysis was G. The results showed that the immune positive products of nRH were not expressed. The results showed that OT had inhibitory effect on GnRH in the hypothalamus pituitary system. The positive products of.GnRH were mainly distributed in spermatogenic cells and support cells of Leydig cells and tubuloseminiferous tubules, and no positive products were expressed in the membrane; the expression of GnRH in testis was higher than that of the control group, and OT There was a very significant difference in the low dose group; the expression of GnRH in the testicular seminiferous tubule decreased with the increase of the dose of OT, and there was a significant difference between the high dose group of OT and the control group. The results showed that OT had a promoting effect on the GnRH expression of the testis and the inhibitory effect on the GnRH of the seminiferous tubule.
OT was intraperitoneally injected into the pre estrus adult female mice, and GnRH was widely distributed in the hypothalamus pituitary ovary axis. The expression of GnRH in the hypothalamus increased significantly compared with the control group. The expression of GnRH in the adenohypophysis was increased compared with the control group, and there was a significant difference in the OT low dose group; the GnRH positive products in the neurohypophysis had not been expressed. The results showed that OT could promote the GnRH performance of the hypothalamus pituitary system. There was no expression of positive products in the membrane, cortex and medulla of the ovary, but the presence of GnRH immunoreactive products in the corpus luteum and follicle granulosa cells, and the GnRH expression in the corpus luteum and follicle of the ovary decreased as compared with those in the group, and the low dose group of OT decreased. The amplitude was greater than that of OT high dose group. The results showed that OT inhibited ovarian GnRH expression.
In the adult mice, the female group, the female and the male group, and the male and female administration group were intraperitoneally injected with OT to mate with the cage. The results showed that the female / male ratio of the female to the different dose groups was higher than that of the control group, and the high dose group was significantly different from the control group. The female / male ratio in the different dose groups was in the male and the male and male groups. The results suggested that exogenous OT promoted the female offspring of female offspring, and the effect depended on the dosage and the way of administration. The vaginal injection of OT to adult female mice showed that the female / male ratio of each dose group was higher than that of the control group, and the relationship with the dose was positive. The results showed that the female / male ratio of the mice was with OT. The dose increase has an upward trend, suggesting that OT acting on female mice is beneficial to the offspring of female offspring.

【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R33

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