鼠防御素cryptdin 4在小鼠内脏分布及其肠炎模型中表达规律

发布时间：2018-04-21 06:03

本文选题：cryptdin + 4　；参考：《吉林大学》2007年硕士论文

【摘要】： 防御素是小分子阳离子抗菌肽,他们既是先天性免疫的效应因子,也参与获得性免疫的调节。目前对于哺乳动物抗菌肽基因的作用及其机制还不清楚。本研究通过检测cryptdin 4在小鼠体内的分布情况及其在肠炎模型中小鼠回肠段cryptdin4基因表达量水平的变化,为研究cryptdin 4作用机制提供理论基础。我们选择了对小鼠回肠、气管、输精管、输卵管四个位置进行免疫组化检测。检测结果发现只有在回肠肠腺基底部的潘氏细胞处有阳性结果,其它三处皆为阴性结果,提示鼠cryp 4在小鼠回肠段有所表达。本实验根据GeneBank中公布的cryp 4和小鼠GAPDH序列设计了两对特异性引物,应用荧光定量PCR技术对肠炎模型状态小鼠回肠段cryp 4表达水平进行检测。在灌药的第24h时回肠内cryp 4表达量升高,随着炎症的发展表达量降低;在第4天时表达量降到最低,随后略有回升,但仍然低于正常水平。本实验的结论可以为进一步研究小鼠cryp 4参与机体免疫的作用及其机制提供理论基础。
[Abstract]:Defensins are small cationic antimicrobial peptides, which are not only the effector of innate immunity, but also involved in the regulation of acquired immunity. The role of antimicrobial peptide gene in mammals and its mechanism are unclear. In this study, the distribution of cryptdin _ 4 in mice and the changes of cryptdin4 gene expression in ileum of mice were detected in order to provide a theoretical basis for studying the mechanism of cryptdin _ 4. Four locations of mouse ileum, trachea, vas deferens and fallopian tubes were detected by immunohistochemistry. The results showed that the positive results were found only in the Pans cells at the base of the ileum and the other three were negative. The results suggested that mouse cryp 4 was expressed in the ileum of mice. Two pairs of specific primers were designed according to the cryp _ 4 and mouse GAPDH sequences published in GeneBank. The expression of cryp _ 4 in ileum of mice with enteritis was detected by fluorescence quantitative PCR technique. The expression of cryp _ 4 in ileum increased at 24 h after administration, decreased with the development of inflammation, reached the lowest level on day 4, then increased slightly, but was still below the normal level. The conclusion of this study may provide a theoretical basis for further study on the role of cryp _ 4 in the immune function of mice and its mechanism.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392

【引证文献】