自体血浆和庆大霉素对细胞因子诱导的杀伤细胞免疫表型和活性的影响

发布时间：2018-04-21 21:21

本文选题：CIK细胞 + 细胞培养　；参考：《细胞与分子免疫学杂志》2014年09期

【摘要】：目的探索自体血浆添加量和庆大霉素对培养的细胞因子诱导的杀伤细胞(CIK细胞)免疫表型和活性的影响。方法采用流式细胞术分析自体血浆和庆大霉素对CIK细胞中不同亚型免疫细胞比例的影响,细胞计数、MTT法检测处理后的CIK细胞对HeLa细胞的细胞毒性差异。结果自体血浆实验组与对照组之间不存在显著性差异,而庆大霉素实验组与对照组相比,CD3+细胞、CD56+细胞、CD3+CD8+细胞和CD3+CD56+细胞的数量均下降,差异有统计学意义(P0.05),下降幅度分别为1.4、1.7、1.4和2.5倍。进一步研究发现,庆大霉素实验组与对照组的CIK细胞对HeLa细胞的细胞毒性分别为43.0%和90.0%,两者存在显著性差异(P0.05)。结论自体血浆对CIK细胞不同亚型比例无明显影响,庆大霉素可引起CD3+细胞、CD56+细胞、CD3+CD8+细胞和CD3+CD56+细胞减少,有一定细胞毒性。
[Abstract]:Objective to investigate the effects of autogenous plasma addition and gentamicin on the immunophenotype and activity of cultured cytokine-induced killer cells (CIK cells). Methods the effects of autologous plasma and gentamicin on the proportion of different subtypes of immune cells in CIK cells were analyzed by flow cytometry. The cytotoxicity of treated CIK cells to HeLa cells was detected by cell count assay. Results there was no significant difference between the autologous plasma test group and the control group, but the number of CD56 CD8 cells and CD3 CD56 cells in Gentamicin group was decreased compared with the control group. The difference was statistically significant (P 0.05), with a decrease of 1.4 and 2.5 times, respectively. Further studies showed that the cytotoxicity of gentamicin CIK cells to HeLa cells was 43.0% and 90.0%, respectively, and there was a significant difference between the two groups (P 0.05). Conclusion there is no significant effect of autogenous plasma on the proportion of different subtypes of CIK cells. Gentamicin can induce the decrease of CD3 CD8 cells and CD3 CD56 cells in CD3 cells.
【作者单位】：深圳市合一康生物科技有限公司;南华大学附属第二医院临床与生物细胞治疗研究所;南华大学附属第二医院检验科;
【基金】：深圳市战略新兴产业发展专项资金(CYZZ20130329145313934)
【分类号】：R392

【相似文献】