人乳头瘤病毒58型晚期蛋白L1在大肠杆菌中的表达及纯化的研究

发布时间：2018-04-22 03:32

本文选题：人乳头瘤病毒58型 + L1基因　；参考：《吉林大学》2006年硕士论文

【摘要】：人乳头瘤病毒(Human Papil1omavirus, HPV)是一种引起人类皮肤、粘膜的乳头状瘤或疣,并可导致恶性病变的病原体。研究表明,90%宫颈癌的发生与人乳头瘤病毒感染有关。近年的流行病学研究显示,人乳头瘤病毒58型(HPV58)在中国妇女宫颈癌标本中检出阳性率非常高,尤其是东南省份,HPV58型的感染有上升趋势,与HPV16型的检出率相近,同时二者在宫颈各病变组的检出强度趋势基本一致。由此可见,在我国HPV58型是仅次于HPV16型的高危型别,与宫颈癌发生关系密切。目前,国内外对HPV 16型已做了大量工作,而对HPV58型的研究却较少。基于HPV58型在我国妇女宫颈癌中的特殊位置,本研究克隆并构建了HPV58 L1基因的重组表达载体,并利用原核细胞表达HPV58 L1主要衣壳蛋白,为研制HPV58型基因工程疫苗打下基础。
[Abstract]:Human papillomavirus (HPVs) is a pathogen that causes papilloma or warts in human skin, mucosa and malignant lesions. Studies have shown that 90% of cervical cancer is associated with human papillomavirus infection. Epidemiological studies in recent years have shown that the positive rate of HPV 58 in Chinese women with cervical cancer is very high, especially the infection of HPV58 in southeastern provinces is on the rise, which is similar to that of HPV16. At the same time, the detection intensity of both groups in cervical lesions was basically the same. It can be seen that HPV58 type is the second highest risk type after HPV16 type in China, which is closely related to the occurrence of cervical cancer. At present, a great deal of work has been done on HPV type 16 at home and abroad, but there is little research on HPV58 type. Based on the special position of HPV58 type in cervical cancer of women in China, the recombinant expression vector of HPV58 L1 gene was cloned and constructed in this study. Prokaryotic cells were used to express the main capsid protein of HPV58 L1, which laid the foundation for the development of HPV58 gene engineering vaccine.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：Q78;R392

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