吸烟、饮酒、冷冻对精子顶体完整率的影响

发布时间：2018-04-22 06:11

本文选题：男性不育 + 顶体完整率　；参考：《东北师范大学》2007年硕士论文

【摘要】： 当前,男性不育是困扰许多育龄夫妇的一个世界性问题。临床上,育龄夫妇中10～15%患有不育不孕症,其中男性原因引起的不育约占不育夫妇的一半。很多因素,包括内部因素和外部因素都影响男性生育力。常规精液分析的方法有精子计数、精子正常形态比例。但这些方法对全面了解生育力尚嫌不足,精子顶体内的酶参与精子受孕过程,精子顶体内的酶含量降低会引起精子生育力下降。精子顶体的完整性能直接反映精子顶体内酶含量的多少,因顶体不完整的精子顶体内酶会漏出,影响精子和卵子的受精。国内外对于顶体完整与受精能力的关系的报道较多,然而国内外对顶体不完整机理研究还很不深入。顶体的不完整究竟是发生在什么部位,是睾丸生成时,还是在附睾中成熟中抑或是在女性生殖道中出现的问题,尚且不得而知,而且究竟是什么外界因素(pH值、温度,及其它小分子和大分子等等)导致了顶体不完整,什么遗传因素导致了顶体出现问题还没有一个令大多数人信服的观点。本文目的是探讨顶体完整率与男性不育关系,通过对488例不育男性精液标本检测,分析常规精液参数与顶体完整率的关系到吸烟、饮酒对顶体完整率的影响,还探讨了辅助生殖技术中优选和冷冻对顶体完整率的影响。采用计算机自动检测系统下人工修正方法进行精子形态分析。患者自愿填写问卷,对吸烟、饮酒因素进行调查分析。结果如下: ①精子形态异常组精子顶体完整率低于精子形态正常组,两组比较有显著性差异(P0.05)。②精液pH值异常组顶体完整率明显低于精液pH值正常组,两组比较有显著性差异(P 0.05)。③精液量正常组和精液量异常组精子顶体完整率进行分析,两组之间比较无显著差异(P 0.05)。④液化时间正常组与液化时间异常组的顶体完整率进行分析,两组之间比较无显著差异(P 0.05)。⑤与生育组比较,吸烟组顶体完整率低于生育组的顶体完整率,两组比较有显著性差异(P 0.01);与不吸烟组比较,吸烟组顶体完整率低于生育组的顶体完整率,两组比较有显著性差异(P 0.05)。⑥与生育组比较,饮酒组顶体完整率低于不吸烟组的顶体完整率,两组比较有显著性差异(P 0.05);与不饮酒组比较,饮酒组的顶体完整率与不饮酒组的顶体完整率没有显著性差异(P 0.05)。⑦与优选前比较,优选后的顶体完整率高于优选前,两组比较有极显著性差异(P 0.001)。⑧与冷冻前比较,快速冷冻和慢速冷冻后顶体完整率与冷冻前比较均没有显著性差异(P 0.05),快速冷冻与慢速冷冻后,两组之间比较亦没有显著性差异(P 0.05)。通过比较研究,得到以下结论:形态正常精子百分率高的精子顶体完整率也高,精子顶体完整率与精子形态具有一致性。精液pH值异常的精子顶体完整率较低,提示pH值影响精子顶体完整率。精液量不影响精子顶体完整率;精液液化时间不影响精子顶体完整率。吸烟能导致顶体完整率降低;饮酒在一定程度上能使精子顶体完整率下降。上游法优选能较好地提高精子顶体完整率。在甘油作为保护剂的情况下,快速冷冻和慢速冷冻都不影响精子顶体完整率。
[Abstract]:At present, male infertility is a worldwide problem that perplex many couples of childbearing age. In clinical, 10 to 15% of the couples of childbearing age have infertility, of which male infertility accounts for half of the infertile couples. Many factors, including internal and external factors, affect male fertility.
The methods of routine semen analysis include sperm count, the proportion of normal sperm morphology. But these methods are not enough to understand the fertility of the sperm in a comprehensive way. The enzyme in the sperm top body participates in the process of sperm pregnancy, and the decrease in the content of the enzyme in the sperm top will cause the decrease of sperm fertility. The finishing performance of the acrosome of the sperm directly reflects the amount of the enzyme in the sperm top body. Less, because the top body incomplete sperm enzyme in the top of the body will leak, affecting the fertilization of sperm and eggs.
There are many reports on the relationship between the integrity of the acrosome and the ability of fertilization at home and abroad. However, the research on the incomplete mechanism of the acrosome is still not thorough. What external factors (pH, temperature, and other small molecules and macromolecules and so on) have led to incomplete acrosome, and what genetic factors lead to the emergence of the acrosome have not yet been convinced by most people.
The purpose of this paper is to investigate the relationship between the integrity of the acrosome and male infertility. Through the examination of the semen specimens of 488 male infertility cases, the influence of the conventional semen parameters and the acrosome integrity rate on the integrity of the acrosome and the effect of drinking on the integrity of the acrosome, and the effect of the optimization and freezing on the integrity of the acrosome in the assisted reproductive technology are also discussed. The artificial correction method was used to analyze the sperm morphology. The patients voluntarily filled in the questionnaire and investigated the factors of smoking and drinking.
(1) the sperm acrosome integrity rate in abnormal sperm morphologic group was lower than that of normal sperm morphology group, and there was significant difference between the two groups (P0.05). The complete rate of acrosome in the abnormal semen pH value group was significantly lower than that of the semen pH normal group, and the two groups were significantly different (P 0.05). There was no significant difference between the two groups (P 0.05). (4) the acrosome integrity of the normal group and the abnormal group of liquefaction time was analyzed, and there was no significant difference between the two groups (P 0.05). 5. Compared with the fertility group, the integrity rate of the acrosome in the smoking group was lower than the acrosome integrity rate in the fertility group, and the two groups had significant differences (P 0.01); and the non smoking group was compared with the non smoking group. Comparison, the integrity rate of the acrosome in the smoking group was lower than the acrosome integrity of the fertility group, and there was a significant difference between the two groups (P 0.05). 6. Compared with the fertility group, the acrosome integrity rate of the drinking group was lower than the acrosome integrity of the non smoking group, and the two groups had a significant difference (P 0.05); the acrosome integrity rate of the drinking group and the acrosome in the non drinking group was compared with the non drinking group. There was no significant difference in the integrity rate (P 0.05). (1) compared with the prior selection, the complete rate of the acrosome after optimization was higher than before the optimization (P 0.001). Compared with pre freezing, there was no significant difference between the acrosome integrity rate of fast freezing and slow freezing before freezing (P 0.05), rapid freezing and slow freezing, two There was no significant difference between the groups (P 0.05).
Through comparative study, we get the following conclusion: the complete rate of sperm acrosome with high percentage of normal sperm is high, the integrity rate of sperm acrosome is consistent with sperm morphology. The intact rate of sperm acrosome with abnormal semen pH value is low, suggesting that pH value affects the integrity of sperm acrosome. Influence of sperm acrosome integrity. Smoking can lead to a decrease in the integrity of the acrosome; drinking to a certain extent can reduce the integrity of the acrosome. Upstream optimization can better improve the integrity of the acrosome. In the case of glycerol as a protective agent, rapid freezing and slow freezing do not affect the integrity of the acrosome.

【学位授予单位】：东北师范大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R321

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