预测并验证HSV-I基因组中的microRNA

发布时间：2018-04-23 00:23

本文选题：microRNA + HSV-I　；参考：《中国协和医科大学》2007年硕士论文

【摘要】： microRNA(miRNA)，即微小RNA，是一类长约22个核苷酸的小分子非编码RNA，通常与靶基因mRNA的3’UTR发生不完全互补，在转录后水平调节基因的表达。自2000年首次报道以来，对microRNA的研究进展迅速，全世界有很多研究小组致力于研究microRNA在各个物种间的分布情况和它所发挥的重要作用。近几年的研究表明，这是一种高度保守的、广泛存在于植物、动物、病毒中的调节机制，，在生长发育、器官的形成、癌症的发生和病毒感染等过程中扮演重要的角色。microRNA的发现是对经典的遗传学法则的重要补充。 HSV-I(herpes simplex virus 1)是一个有包膜的双链DNA病毒，具有庞大的基因组，目前已知的编码蛋白有84个，分为α、β、γ三类，基因表达的调控极为复杂，很多基因的功能以及调控过程仍不清楚。HSV-I在原发感染之后不能被宿主免疫系统彻底清除，会终生潜伏在宿主的神经节细胞内，一定状态下被重新激活，对机体造成损伤。目前的研究结果仍不能很好地解释这种特殊的感染-潜伏-再激活的感染过程。本课题的目的是预测并验证HSV-I基因组中新的micmRNA，进而研究它在HSV-I基因表达调控中的作用。利用生物信息学的方法，依据一定的条件，我们确定了HSV-I基因组中可能编码microRNA的27个茎环结构，其中有4个在microRNA序列比对和预测软件miRBase∷Sequences中显示出较高的得分且在Herpes病毒家族中较为保守。提取HSV-I感染的细胞small RNA后，经Northern blot分析，结果显示，α基因ICP0的内含子区编码一个新的microRNA—mihsv-2。在对其功能进行了初步的分析后，认为mihsv-2很可能影响人的基因Kelch like-24的表达。本实验尝试从新的角度研究HSV-I基因表达的调控机制，希望为今后的研究提供有用的线索。
[Abstract]:MicroRNAs miRNAs, or microRNAs, are a class of small non-coding RNAs with about 22 nucleotides in length, which are usually not fully complementary to the 3'UTR of the target gene mRNA, and regulate the expression of genes at the post-transcriptional level. Since it was first reported in 2000, the research on microRNA has made rapid progress. There are many research groups all over the world devoted to studying the distribution of microRNA among species and its important role. Studies in recent years have shown that this is a highly conservative, widespread regulatory mechanism in plants, animals and viruses, in growth and development, in organ formation, The discovery of microRNA plays an important role in the development of cancer and viral infection. HSV-I(herpes simplex virus 1 (HSV-I(herpes simplex virus 1) is a double-stranded DNA virus with a large genome. At present, there are 84 known encoded proteins, which are classified into three types: 伪, 尾, 纬, and the regulation of gene expression is very complex. The function and regulatory process of many genes are still unclear. HSV-I can not be completely eliminated by host immune system after primary infection. HSV-I will lurk in the host's ganglion cells for life and be reactivated in a certain state, causing damage to the body. Current research results still do not explain this particular infection-latent-reactivation infection process. The purpose of this study is to predict and verify new micmRNAs in the HSV-I genome and to study its role in the regulation of HSV-I gene expression. Using the bioinformatics method and according to certain conditions, we have identified 27 stem ring structures in the HSV-I genome that may encode microRNA. Four of them showed higher scores in microRNA sequence alignment and prediction software miRBase: Sequences and were more conservative in the Herpes virus family. After extracting small RNA from HSV-I infected cells, the results of Northern blot analysis showed that the intron region of 伪 gene ICP0 encodes a new microRNA-mihsv-2. After a preliminary analysis of its function, it was suggested that mihsv-2 might affect the expression of human gene Kelch like-24. This study attempts to study the regulatory mechanism of HSV-I gene expression from a new perspective, hoping to provide useful clues for future research.
【学位授予单位】：中国协和医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R373

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