ZHJ-MAPCs的细胞学初步分析鉴定及共培养诱导向肝样细胞分化研究

发布时间：2018-04-25 06:48

本文选题：ZHJ-MAPCs + 细胞传代　；参考：《第一军医大学》2005年硕士论文

【摘要】：治疗终末期肝病最有效的方法之一就是原位肝移植(orthotopic liver transplantation,OLT),然而,由于供体的缺乏、免疫排斥和高额费用等问题,OLT的广泛应用受到了限制。肝细胞移植(hepatocytes transplantation HCT)经过近30年的发展,正由动物实验向临床过渡,初步结果显示,肝细胞移植在急慢性肝衰竭和遗传代谢性肝病方面具有很好的应用前景,但同样面临肝细胞来源有限,排斥反应等问题的困扰。杂交型生物人工肝(Hybrid bioartificial liver,HBAL)治疗可以使终末期肝病病人桥接(bridge)肝移植,而且由于肝细胞具有强大的再生能力,临时应用生物人工肝也可以暂时代偿肝功能,促进肝细胞再生,部分患者肝功能完全恢复正常。但作为HBAL核心生物材料的肝细胞来源问题未得到根本解决,使HBAL的临床应用远未达到理论上的预期效果。研究证明人骨髓来源的多能成体祖细胞(Multipotent adult progenitor cells from bone marrow,MAPCs)具有向肝样细胞分化的潜能,由于其具有易于增殖、调控、供源丰富、容易获取、有自体供源避免免疫排斥等优点,有望成为理想的组织工程细胞来源。本课题组前期实验通过密度梯度离心—贴壁培养筛选—免疫磁性双阴性分选(magnetic activated cell sorting,MACS)三个步骤高效率地纯化分选出骨髓间充质干细胞(mesenchymal stem cells,MSCs)亚群MAPCs(CD45~-,GlyA~-),并将之命名为ZHJ-MAPCs(Zhujiang-MAPCs)。通过改良细胞培养基等方法,我们建立了ZHJ-MAPCs的传代培养技术(结果尚未发表)。
[Abstract]:One of the most effective methods for the treatment of end-stage liver disease is in situ orthotopic liver transplantation (OLT). However, the wide application of OLT is limited due to the lack of donor, immune rejection and high cost. The development of hepatocytes transplantation HCT (hepatocytes transplantation HCT) is being conducted by animal experiments for nearly 30 years. The preliminary results show that hepatocyte transplantation has a good application prospect in acute and chronic liver failure and genetic metabolic liver disease, but it also faces problems such as limited source of hepatocyte and rejection. Hybrid bioartificial liver (HBAL) can bridge patients with end-stage liver disease (Bridg E) liver transplantation, and because of the powerful regeneration ability of liver cells, temporary application of biological artificial liver can also temporarily compensate liver function, promote hepatocyte regeneration, and restore normal liver function in some patients. However, the liver cell source problem of HBAL core biomaterial has not been fundamentally solved, so that the clinical application of HBAL is far from the theory. Multipotent adult progenitor cells from bone marrow, MAPCs) has the potential to differentiate into hepatocyte like cells. It is expected to be an ideal tissue because it has the advantages of easy proliferation, regulation, rich source, easy access, and autologous source to avoid immune rejection. The project cell source. In the preliminary experiment, we purified the bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) subgroup MAPCs (CD45~-, GlyA~-) by the three steps of the density gradient centrifugation - adherent culture screening - magnetic activated cell sorting (MACS). PCs (Zhujiang-MAPCs). By means of improved cell culture medium, we have established ZHJ-MAPCs subculture Technology (the result has not yet been published).

【学位授予单位】：第一军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R329.2;R657.3

【参考文献】