整合子—基因盒系统介导和传播耐药性的研究

发布时间：2018-04-25 21:32

  本文选题：大肠埃希菌 + 整合子　； 参考：《四川大学》2005年硕士论文

【摘要】：目的 监测大肠埃希菌临床分离株的耐药性,了解大肠埃希菌中整合子的存在频率、类型,以及基因盒的种类和排列,观察细菌携带基因盒与耐药表型的关系,以及整合子在不同基因型菌株中的分布,探讨整合子-基因盒系统介导和传播细菌耐药性的分子机制。 方法 利用全自动细菌分析仪,对94株大肠埃希菌临床分离株进行确认、产ESBLs判读和20种抗生素敏感性测定,纸片扩散法对另外4种抗生素进行敏感性测定;应用PCR方法,扩增总DNA和质粒DNA上Ⅰ类整合子、Ⅱ类和Ⅲ类整合酶;对大小相同的Ⅰ类整合子进行酶切分析;对纯化后的Ⅰ类整合子进行DNA测序,将DNA序列在GeneBank中搜索,确定Ⅰ类整合子可变区基因盒的种类和排列;利用脉冲场电泳(PFGE)对大肠埃希菌进行分子分型。 结果 细菌对15种抗生素的耐药率超过50%,耐药率高的抗生素包括氨苄青霉素(94%)、氧哌嗪青霉素(87%)等,45株(47.9%)细菌产ESBLs,90株(95.7%)细菌为多重耐药菌(耐受3种以上
[Abstract]:Purpose To monitor the drug resistance of clinical isolates of Escherichia coli, to understand the frequency and type of integron in Escherichia coli and the type and arrangement of gene boxes, and to observe the relationship between bacterial cassette and phenotype of drug resistance. And the distribution of integron in different genotypes, and the molecular mechanism of integron-gene box system mediated and spread bacterial drug resistance. Method Using automatic bacterial analyzer, 94 clinical isolates of Escherichia coli were confirmed, ESBLs interpretation and 20 antibiotic sensitivity tests, disk diffusion method were used to determine the sensitivity of the other 4 antibiotics, and PCR method was used. The class 鈪，

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