小鼠甘露糖受体中CR结构域的表达、纯化及其功能的初步研究

发布时间：2018-04-26 03:07

本文选题：CR-Fc + 嵌合蛋白　；参考：《大连医科大学》2006年硕士论文

【摘要】： 甘露糖受体(MR)为甘露糖受体家族中的一员,属于C-型凝集素受体。甘露糖受体广泛分布于体内的各种组织,主要表达于髓样细胞系,包括非成熟的树突状细胞和巨噬细胞亚群。此外,肝的窦状隙细胞,人的表皮朗罕氏细胞,肾小球系膜细胞,内皮细胞亚群,气管平滑肌细胞,视网膜上皮细胞等都发现了甘露糖受体的表达。甘露糖受体在调节内环境的稳定,介导先天性免疫及获得性免疫应答中都有其明确的作用。此外,甘露糖受体可能参与了肿瘤的淋巴道转移以及肿瘤微环境中肿瘤的免疫逃避。甘露糖受体为相对分子量为175kDa的一种I型跨膜受体,由五部分组成:N-末端富含半胱氨酸结构域(CR);II型纤连蛋白重复序列(FNII);8个C-型凝集素样结构域(CTLDs);跨膜结构域;C末端的胞质结构域.。甘露糖受体能够通过不同的结构域与内源性以及外源性配体结合。富含半胱氨酸结构域(CR)为甘露糖受体的N端序列,专门识别内源性的酸性聚糖,能够与糖蛋白末端的4-硫酸化,3-硫酸化的N-乙酰基葡萄糖胺以及3-硫酸化的半乳糖结合。体内的多种激素,例如,垂体激素,促黄体生成素,促甲状腺激素都表达该硫酸化的结构。此外,在淋巴结组织中发现了富含半胱氨酸结构域的多种配体,包括唾液酸黏附素(Sn),CD45以及一些有待于进一步鉴定的潜在配体。 L-选择素在正常情况下为淋巴细胞的归巢受体.淋巴细胞表面的黏附分子与淋巴管内皮细胞通过多环节的相互作用介导了淋巴细胞的归巢过程。其中淋巴细胞表达的L-选择素在此过程中发挥了重要的作用,L-选择素通过与HEVs表面表达的外周淋巴结黏附素(PNAds)相互作用而进入淋巴系统。近来研究发现,人的输出淋巴管内皮有MR的表达,而且淋巴细胞可能通过表达的L-选择素与输出淋巴管内皮表达
[Abstract]:Mannose receptor MRA is a member of mannose receptor family and belongs to C-type lectin receptor. Mannose receptors are widely distributed in various tissues, mainly expressed in myeloid cell lines, including immature dendritic cells and macrophage subsets. In addition mannose receptor expression was found in sinusoid space cells of liver human epidermal Langerhans cells glomerular Mesangial cells endothelial cell subsets tracheal smooth muscle cells and retinal epithelial cells. Mannose receptors play an explicit role in regulating the stability of the internal environment and mediating innate and acquired immune responses. In addition, mannose receptors may be involved in lymphatic metastasis and immune evasion in tumor microenvironment. Mannose receptor is a type I transmembrane receptor with relative molecular weight of 175kDa. It is composed of five parts: the cysteine-rich domain (CRN) type II fibronectin repeats (FNIIs), eight C-type lectin-like domains (CTLDsN), and the cytoplasmic domain (CTLDsN) at the C-terminal of the transmembrane domain. Mannose receptors can bind to endogenous and exogenous ligands through different domains. Cysteine rich domain (CRR) is a N-terminal sequence of mannose receptors, which specifically recognizes endogenous acidic glycans and binds to 4-sulfated 3-sulfated N-acetylglucosamine and 3-sulfated galactose at the end of glycoprotein. Many hormones in the body, such as pituitary hormones, luteinizing hormone, and thyroid stimulating hormone, all express the sulfated structure. In addition, various ligands rich in cysteine domain have been found in lymph node tissues, including sialic acid adhesin (SNP) CD45 and some potential ligands for further identification. L-selectin is normally the homing receptor of lymphocytes. Lymphocyte homing is mediated by adhesion molecules on lymphocyte surface and lymphatic endothelial cells. L- selectin expressed by lymphocytes plays an important role in this process. L- selectin enters the lymphatic system by interacting with peripheral lymph node adhesin (PNAds) expressed on the surface of HEVs. Recent studies have found that human output lymphatic endothelium is expressed by Mr, and lymphocytes may be expressed by expression of L- selectin and output lymphatic endothelium.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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