人卵泡抑素及MUC1的组织学表达分析

发布时间：2018-04-27 03:15

本文选题：卵泡抑素 + MUC1　；参考：《吉林大学》2007年硕士论文

【摘要】： 肿瘤是严重威胁人类健康和生命的疾病之一,其发生、发展是多因素、多步骤、多阶段的复杂过程,不同分期、分型肿瘤选择的治疗方式不同,早发现、早治疗是肿瘤诊治的重要原则。卵泡抑素(follistatin,FS)是一种单链糖蛋白,具有广泛的组织分布及多种生物学活性。粘蛋白1(MUC1)是mucin粘蛋白家族成员,存在于正常腺管上皮细胞及其来源的肿瘤细胞中。FS、MUC1在肿瘤诊断、预后判断等方面具有重要意义。为进一步探讨FS及MUC1在肿瘤诊断方面的临床意义,本实验利用双位点ELISA法检测肿瘤患者外周血中FS蛋白水平变化;荧光定量RT-PCR检测不同类型肺癌组织、肺癌及乳腺癌患者外周血循环FS及MUC1 mRNA水平变化;采用免疫组织化学染色检测FS、MUC1在不同病理类型卵巢癌、乳腺癌及肺癌组织内的分布情况。研究结果显示,卵巢癌、肝癌、乳腺癌、肺癌患者外周血FS蛋白水平明显高于健康对照组;除小细胞肺癌外周血MUC1 mRNA无明显变化外,肺癌组织和外周血循环FS mRNA及外周血MUC1 mRNA水平明显高于对照组,p0.01;乳腺癌患者外周血MUC1 mRNA水平明显高于对照组,p0.05。免疫组化研究结果表明,卵巢肿瘤组织抗FS抗体组化染色强度依次为粘液性癌子宫内膜样癌鳞癌透明细胞癌浆液性囊腺癌,抗MUC1抗体组化染色强度各肿瘤间无明显差异;肺癌组织抗FS抗体组化染色强度依次为腺癌腺鳞癌鳞癌未分化小细胞癌。为研究FS、MUC1与妊娠的关系,本实验同时观察了妊娠早期及难免流产患者外周血循环FS及MUC1 mRNA水平及胎盘绒毛组织FS、MUC1表达情况。难免流产和正常妊娠比较,其绒毛组织、外周血中FS mRNA、MUC1 mRNA水平均显著下降;妊娠早期胎盘绒毛组织FS、MUC1蛋白表达水平明显高于难免流产胎盘绒毛组织。本研究资料提示FS在不同组织来源的肿瘤细胞表达水平不同,在同一组织来源的不同病理类型的肿瘤细胞表达水平也不同。肿瘤患者外周血FS、MUC1 mRNA水平升高,可能直接来源于肿瘤细胞,对肿瘤诊断具有重要意义。绒毛组织的FS及MUC1水平变化提示FS、MUC1与生理病理妊娠有关,可能在维持正常妊娠中具有重要作用,但其机制有待进一步研究。
[Abstract]:Cancer is one of the serious threats to human health and life. Its occurrence and development is a complex process of multi-factor, multi-step, multi-stage, different stages, different treatment methods of tumor classification, and early detection. Early treatment is an important principle of tumor diagnosis and treatment. Follistatin (FS) is a single chain glycoprotein with a wide range of tissue distribution and a variety of biological activities. Mucin 1 (MUC1) is a member of mucin mucin family. In order to further explore the clinical significance of FS and MUC1 in the diagnosis of tumor, we used double locus ELISA method to detect the changes of FS protein in peripheral blood of tumor patients, and fluorescence quantitative RT-PCR was used to detect different types of lung cancer tissues. The levels of circulating FS and MUC1 mRNA in peripheral blood of patients with lung cancer and breast cancer were determined by immunohistochemical staining, and the distribution of FAS MUC1 in different pathological types of ovarian cancer, breast cancer and lung cancer was detected by immunohistochemical staining. The results showed that the levels of FS protein in peripheral blood of patients with ovarian cancer, liver cancer, breast cancer and lung cancer were significantly higher than those of healthy controls, except for the small cell lung cancer patients with no significant changes in peripheral blood MUC1 mRNA. The levels of FS mRNA and MUC1 mRNA in lung cancer tissue and peripheral blood circulation were significantly higher than those in control group (p 0.01), and the levels of MUC1 mRNA in breast cancer patients were significantly higher than those in control group (P 0.05). The results of immunohistochemical study showed that the intensity of anti-FS antibody histochemical staining in ovarian tumors was mucinous carcinoma, endometrial carcinoma, clear cell carcinoma, serous cystadenocarcinoma, and there was no significant difference in the intensity of anti-FS antibody histochemical staining among the tumors. The histochemical staining intensity of anti FS antibody in lung cancer was adenocarcinoma, adenosquamous carcinoma, squamous cell carcinoma, undifferentiated small cell carcinoma. In order to study the relationship between FSS-MUC1 and pregnancy, the levels of circulating FS and MUC1 mRNA in peripheral blood and the expression of MUC1 in placental villi in early pregnancy and inevitable abortion were observed. Compared with normal pregnancy, the level of FS mRNA-MUC1 mRNA in villi and peripheral blood decreased significantly, and the expression level of FSmRNA-MUC1 protein in placental villi in early pregnancy was significantly higher than that in placental villi of inevitable abortion. The data suggested that the expression level of FS was different in tumor cells from different tissues and different pathological types of tumor cells from the same tissue. The elevated level of MUC1 mRNA in peripheral blood of tumor patients may be directly derived from tumor cells, which is of great significance for the diagnosis of tumor. The changes of FS and MUC1 in chorionic villi suggest that MUC1 may play an important role in maintaining normal pregnancy, but its mechanism needs further study.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392

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