痰阻血脉大鼠模型的建立及固本化痰通脉方作用的研究

发布时间：2018-04-28 02:39

本文选题：痰 + 高脂血症　；参考：《湖北中医学院》2006年硕士论文

【摘要】：目的本文在食饵法高脂血症动物模型的基础上建立痰阻血脉大鼠模型，通过观察大鼠血浆血脂的变化来评价模型。通过观察主动脉内皮巨噬细胞与粘附分子的表达及巨噬细胞的形态，来探讨痰邪致病的细胞和分子机制。同时，观察固本化痰通脉方对模型大鼠的影响，，以反证模型成功与否，以及对痰证的治疗作用，为临床治疗痰证提供一定依据。方法 7周龄健康雄性Wistar大鼠50只，200±20g，随机分为5组：正常组、模型组、固本化痰通脉方低剂量组(简称固低组)、固本化痰通脉方高剂量组(简称固高组)、西药组。观察大鼠的一般情况，包括饮食情况、活动度、毛发光泽度等变化；处死后观察各组大鼠血浆中TC、TG、HDL、LDL的变化；采用免疫组织化学方法检测主动脉内皮MΦ、ICAM-1、VCAM-1、P-selectin、E-selectin的表达，并经图像分析定量；运用透射电镜观察主动脉内皮巨噬细胞的超微结构。结果模型组大鼠普遍出现纳差、少动、拱背嗜睡、毛色发黄、缺乏光泽及掉毛严重等表现。正常组大鼠活泼好动、毛色光亮、食欲旺盛。模型组血脂水平与正常组相比具有显著性差异(P＜0.01)；经治疗后，TC、TG、LDL-c降低，HDL-c升高；与模型组相比，固低组TG、LDL-c降低，HDL-c升高有差异(P＜0.05)，TC降低有显著性差异(P＜0.01)；固高组和西药组与之均有显著性差异(P＜0.01)。与西药组相比，固高组降TG有差异(P＜0.05)，其余无显著性差异；固低组TC、HDL-c、LDL-c具显著性差异(P＜0.01)，TG无显著性差异。与固低组比，固高组TC、LDL-c有差异(P＜0.05)，TG、HDL-c有显著性差异(P＜0.01)。免疫组化结果显示，正常组几乎没有巨噬细胞表达，模型组与之相比有显著性差异(P＜0.01)，固低组与西药组无显著性差异(P＞0.05)，固高组与二者有显著性差异(P＜0.01)；模型组ICAM-1、VCAM-1、P-selectin、E-selectin的表达升高，与正常组相比有显著性差异(P＜0.01)，经治疗后表达降低，与固高组相比有显著性差异(P＜0.01)。超微结构结果显示模型组血管内皮下内膜水肿、增宽，甚至间断不完整；血中单核细胞及淋巴细胞向内弹力板浸润，可见较多巨噬细胞，其胞浆内含大量各级溶酶体及吞噬小泡，内质网及核糖体增多，胞膜外出现伪足；内皮细胞形态发生改变，可见线粒体水肿，胞浆不均匀；中药治疗后，上述病理损害明显减轻。
[Abstract]:Purpose Based on the animal model of hyperlipidemia with prey method, the rat model of phlegm blocking blood vessel was established, and the model was evaluated by observing the changes of plasma lipids in rats. The expression of endothelial macrophages and adhesion molecules and the morphology of macrophages were observed to explore the cellular and molecular mechanisms of phlegm pathogen. At the same time, to observe the effect of Guben Huatan Tongmai recipe on the model rats, to reverse the success of the model, as well as the treatment of phlegm syndrome, to provide a certain basis for clinical treatment of phlegm syndrome. Method A total of 50 healthy male Wistar rats aged 7 weeks were randomly divided into 5 groups: normal group, model group, Guben Huatan Tongmai prescription low dose group, Guben Huatan Tongmai prescription high dose group (referred to as Gugao group, western medicine group). The changes of diet, activity, hair glossiness and so on were observed, the changes of plasma TCU TGG HD-LDL and the expression of P-selectin in aorta endothelium M 桅 ICAM-1 VCAM-1 and P-selectin were detected by immunohistochemical method. The ultrastructure of aortic endothelial macrophages was observed by transmission electron microscope. Result In the model group, anorexia, motility, sleepiness, yellowish coat color, lack of luster and severe hair loss were found. Normal group rats were lively and active, bright coat color and strong appetite. There was significant difference in blood lipid level between the model group and the normal group (P < 0.01). There was significant difference between the two groups (P < 0.05) in decreasing HDL-c and the difference between the two groups (P < 0.01), and there was a significant difference between the two groups (P < 0.01). Compared with the western medicine group, there was significant difference in TG reduction between the Gugao group and the western medicine group (P < 0.05), but there was no significant difference in TG between the two groups (P < 0.01), but there was no significant difference between the two groups (P < 0.01). There was significant difference (P < 0.05) in LDL-c of TCU and HDL-c between the two groups (P < 0.01). The results of immunohistochemistry showed that there was almost no macrophage expression in normal group, there was significant difference between model group and model group (P < 0.01), there was no significant difference between Gu-low group and western medicine group (P > 0.05), there was significant difference between Gugao group and both groups (P < 0.01), and the expression of ICAM-1VCAM-1P-selectinin E-selectin was increased in model group. Compared with the normal group, there was a significant difference (P < 0.01), the expression decreased after treatment, and there was significant difference (P < 0.01) compared with the Gugao group. The ultrastructural results showed that the intima of vascular endothelium in the model group was edematous, enlarged, and even discontinuous incomplete, the monocytes and lymphocytes in the blood infiltrated into the elastic plate, and more macrophages were observed. Its cytoplasm contains a large number of lysosomes and phagocytic vesicles, the endoplasmic reticulum and ribosome increase, and pseudopodia appear outside the cell membrane. The above pathological lesions were obviously alleviated.
【学位授予单位】：湖北中医学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332;R285

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