立体定向毁损边缘多巴胺系统的实验研究

发布时间：2018-04-28 12:54

本文选题：立体定向 + 伏隔核　；参考：《青岛大学》2007年硕士论文

【摘要】： 目的：研究多靶点联合毁损边缘多巴胺系统对大鼠认知功能的影响，从而为立体定向手术筛选出安全的手术靶点。方法：选用成年Spraqui-Dawlay大鼠50只，其中40只作为毁损组，5只作为对照组，5只作为假手术组。毁损组又分为伏隔核毁损组(毁损部位：扣带回+伏隔核)、杏仁核毁损组(毁损部位：扣带回+杏仁核基底外侧核)、海马毁损组(毁损部位：扣带回+海马CA3区)、隔区毁损组(毁损部位：扣带回+隔区)，每组10只。应用直流电极立体定向技术同期对称毁损大鼠不同靶点，利用组织学方法确定毁损部位及范围，术后第1周、2周进行利用Morris水迷宫定位航行试验和空间探索试验评估手术对大鼠认知功能的影响。结果：在成功准确毁损边缘多巴胺系统的靶点后，Morris水迷宫结果显示：伏隔核毁损组术后第1周大鼠寻找平台的逃避潜伏期延长，由10.1±1.2s延长至13.2±1.4s，具有明显统计学差别：(t=16.24，P＜0.01)；第2周潜伏期缩短为9.6±0.9s，潜伏期短于术前水平，差别具有显著性：(t=5.31，P＜0.01)。伏隔核组术后第1周在平台象限内停留的时间由术前41.5±3.3s缩短为术后35.1±2.8s，差别具有显著性：(t=4.30，P=0.004)；术后第2周则恢复到术前水平。综上所述，伏隔核组大鼠术后认知功能有短暂的下降，但很快恢复至正常或高于正常水平。杏仁核组结果分析表明与伏隔核组相似，，术后有短暂的下降，很快恢复正常。海马毁损组则显示术后逃避潜伏期明显延长，探索时问明显缩短，都具有统计学差异，并且在术后第2周无明显变化。隔区毁损与海马相似，但潜伏期延长和探索时间缩短程度都小于海马组。结论：1、杏仁核毁损对大鼠认知功能无明显影响；伏隔核毁损在术后可以出现短暂的认知功能下降，但很快恢复至术前水平；海马和隔区毁损大鼠可以出现认知功能的近期和远期下降(至少是2周内)。对认知功能的影响而言，依据作用大小依次排列为：海马＞隔区＞伏隔核、杏仁核。2、杏仁核毁损是相关立体定向手术的安全、有效的靶点；伏隔核组毁损则可作为相关手术的备用靶点；而海马和隔区毁损可严重影响大鼠认知功能，不能作为手术靶点。
[Abstract]:Aim: to study the effects of multiple targets combined with lesion of edge dopamine system on cognitive function in rats, and to screen safe targets for stereotactic surgery. Methods: 50 adult Spraqui-Dawlay rats were selected, of which 40 were used as lesion group (n = 5) and 5 as control group (n = 5) as sham operation group. The lesion group was divided into two groups: nucleus accumbens lesion group (lesion site: cingulate nucleus accumbens nucleus), amygdaloid nucleus lesion group (lesion site: basal lateral nucleus of cingulate gyrus amygdala nucleus), hippocampal lesion group (lesion site: cingulate cortex hippocampal CA3 region). The septal lesion group (lesion site: cingulate gyrus septum, 10 rats in each group). The stereotactic technique of DC electrode was used to determine the location and range of damage by histological method. The effects of the operation on the cognitive function of rats were evaluated by Morris water maze navigation test and space exploration test 2 weeks after operation. Results: the results of Morris water maze showed that the escape latency of the rats in the nucleus accumbens group was prolonged at the first week after the successful and accurate destruction of the limbic dopamine system. From 10.1 卤1.2 s to 13.2 卤1.4 s, there was a significant difference between the two groups (P < 0.01), and the second week latency was shortened to 9.6 卤0.9 s, which was shorter than the preoperative level (P < 0.01). In the nucleus accumbens group, the time of staying in the platform quadrant was shortened from 41.5 卤3.3 s to 35.1 卤2.8 s in the first week after operation. The difference was significant (P < 0.05), and recovered to the preoperative level at the second week after operation. In conclusion, the cognitive function of rats in nucleus accumbens group decreased briefly after operation, but quickly returned to normal or higher than normal level. The results of amygdaloid nucleus group showed that similar to the nucleus accumbens group, there was a brief decrease after operation and quickly returned to normal. In the hippocampal lesion group, the escape latency was prolonged and the time of exploration was shortened significantly, and there was no significant change in the second week after operation. The septal lesion was similar to that of hippocampus, but the prolongation of latency and the shortening of exploration time were smaller than those of hippocampal group. Conclusion the damage of amygdaloid nucleus has no obvious effect on cognitive function of rats, and the lesion of nucleus accumbens can cause a brief decline of cognitive function after operation, but it can recover to the level of preoperation. Cognitive impairment in the hippocampus and septum may occur in the near and long term (at least within 2 weeks). According to the effect of cognitive function, the order of action is: hippocampus > septum > nucleus accumbens, amygdala. The lesion of amygdala is a safe and effective target for stereotactic surgery. The lesion of nucleus accumbens can be used as a reserve target for related surgery, while the lesion of hippocampus and septum can seriously affect the cognitive function of rats and can not be used as a target for surgery.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R33

【参考文献】