miR-126对EA.hy926细胞血管内皮生长因子表达的调控作用

发布时间：2018-04-28 15:48

本文选题：RNA干扰 + miR-　；参考：《中国病理生理杂志》2014年01期

【摘要】：目的:探讨miR-126对EA.hy926细胞血管内皮生长因子(VEGF)表达的影响。方法:选用EA.hy926细胞株作为研究对象,采用阳离子介导的转染方式,将miR-126 inhibitor和miR-126 mimics进行细胞转染,分析miR-126对血管内皮细胞功能的影响。采用转染negative control作为阴性对照,转染36 h后提取细胞总RNA,利用real-time PCR和Western blotting分别检测EA.hy926细胞VEGF mRNA和蛋白水平的表达。结果:转染miR-126 inhibitor 50 nmol/L 36 h后,EA.hy926细胞的VEGF mRNA及蛋白水平各组间差异有统计学意义(P0.01),与阴性对照组相比,miR-126 inhibitor使EA.hy926细胞的VEGF mRNA及蛋白水平显著上调(P0.01);转染miR-126 mimics 50 nmol/L 36 h后,EA.hy926细胞的VEGF mRNA及蛋白水平各组间有显著差异(P0.01),与阴性对照组相比,miR-126 mimics使EA.hy926细胞的VEGF在mRNA及蛋白水平显著下调(P0.01)。结论:miR-126能够抑制VEGF的表达,VEGF有可能是其调节的靶基因之一。
[Abstract]:Objective: to investigate the effect of miR-126 on the expression of vascular endothelial growth factor (VEGF) in EA.hy926 cells. Methods: miR-126 inhibitor and miR-126 mimics were transfected into EA.hy926 cells by cationic transfection. The effect of miR-126 on the function of vascular endothelial cells was analyzed. Transfection of negative control was used as negative control. Total RNAs were extracted after transfection for 36 h. The expression of VEGF mRNA and protein in EA.hy926 cells were detected by real-time PCR and Western blotting, respectively. Results: the VEGF mRNA and protein levels of EA.hy926 cells transfected with miR-126 inhibitor 50 nmol/L for 36 h were significantly different (P 0.01). Compared with the negative control group, the VEGF mRNA and protein levels of EA.hy926 cells were significantly up-regulated by inhibitor, and the miR-126 mimics 50 nmol/L was transfected for 36 h. There was a significant difference in VEGF mRNA and protein levels in EA.hy926 cells. Compared with the negative control group, miR-126 mimics significantly down-regulated the VEGF of EA.hy926 cells at the level of mRNA and protein. ConclusionVEGF-126can inhibit the expression of VEGF and may be one of its target genes.
【作者单位】：汕头大学医学院附属肿瘤医院药学部;汕头大学医学院附属肿瘤医院内科;
【分类号】：R363

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