含镍金属植入对受孕和胚胎发育及局部致癌的影响

发布时间：2018-04-30 03:10

本文选题：镍 + 大鼠　；参考：《兰州大学》2007年硕士论文

【摘要】： 目的：植入镍(Nickel，Ni)及镍钛(Ni Ti shape memory alloy，NiTi)合金于大鼠后肢股部，模拟人体合金植入，以纯金镍作为高剂量镍离子(Ni~(2+))释放组，合金组为低剂量释放组，观察植入物持续镍离子(Ni~(2+))释放对大鼠生殖和胚胎发育的影响，探讨含镍金属镍离子(Ni~(2+))释放对雌鼠生殖能力的影响，以及含镍金属对植入局部的病理影响和生物学特点，揭示金属镍体内缓慢腐蚀释放对大鼠生殖过程和局部组织的毒理影响，进一步了解含镍金属假体在生物体内缓慢离子释放的生物安全度。方法：大鼠随机分为假手术组(Sham operated group)、镍组(Nickel group)、合金组(Allay group)；分别植入金属镍5g/Kg体重及镍钛合会10g/Kg体重于股部，使镍组和合金组植入镍含量总量相同，配对后分笼常规饲养，观察Ni~(2+)在体内持续释放对大鼠受孕和胚胎发育的影响，计数受孕率、活胎率、死胎率、胎吸收率，计算胎儿体重，重要脏器系数、新生鼠体重增长及对植入部位局部组织的影响，对主要脏器的病理学影响等。结果：镍组大鼠的着床数、活胎率较假手术组和合金组低(P＜0.05)，死胎率高于假手术组和合金组(P＜0.05)，胎鼠的体重和肝、肺、肾脏器系数镍组低于假手术组和合金组(P＜0.05)，生后第1d、5d新生鼠体重镍组低于假手术组和合金组(P＜0.05)，10、15、20d新生鼠各组无显著性差异。局部组织在早期40d时形成炎性肉芽肿及其坏死，中期80d时为局部囊性组织增生和血性渗出液，组织类型为横纹肌水肿，肌纤维紊乱，，粗大，内容物为破碎红细胞及少量淋巴细胞，晚期160d时局部包块增大，囊壁增厚分隔，病理组织类型为胚胎型横纹肌肉瘤，可见大量均匀的深染癌细胞，内容物为大量异常的间皮细胞和散落的癌细胞。胎鼠脏器肉眼解剖均未发现明显结构异常；镍组死亡胎鼠面颅和脑颅均发育较差，存活镍组新生鼠未见异常。光镜可见一例镍组胎鼠视网膜畸形，余组未见异常；股骨头软骨发育镍组较合金组和假手术组差，表现为软骨增殖区同源细胞减少。镍组新生鼠生存能力下降，死亡数增加，体重增长减慢。
[Abstract]:Objective: to implanted nickel (Nickel, Ni) and nickel titanium (Ni Ti shape memory alloy, NiTi) alloy in the femoral part of the hind limbs of rats to simulate human alloy implantation and YISHION gold nickel as a high dose nickel ion (Ni~ (2+)) release group. The alloy group was a low dose release group. The effects of the release of the implanted nickel ions (Ni~ (2+)) on the reproductive and embryonic development of rats were observed. The effects of nickel metal nickel ions (Ni~ (2+)) on the reproductive capacity of female rats, and the pathological and biological characteristics of nickel containing metal to the local implantation, reveal the toxicological effects of the slow corrosion release of nickel metal on the reproductive process and local tissues of the rats, and further understand the birth of the slow ion release of the nickel bearing metal prosthesis in the organism. Safety of things.
Methods: rats were randomly divided into sham operation group (Sham operated group), nickel group (Nickel group) and alloy group (Allay group). The nickel and titanium alloy group were implanted in the femoral part, respectively. The nickel content and the alloy group were implanted in the same amount of nickel in the nickel group and the alloy group, and the normal feeding of Ni~ (2+) in the body was observed. The effects of pregnancy and embryo development, counting the pregnancy rate, the rate of live fetus, the rate of stillbirth, fetal absorption, calculating the fetal weight, the important organ coefficient, the weight growth of the newborn rats and the effect on the local tissue of the implanted parts, and the pathological effects on the main organs.
Results: the number of implantation in the nickel group was lower than that of the sham operation group and the alloy group (P < 0.05). The stillbirth rate was higher than that of the sham operation group and the alloy group (P < 0.05). The weight of the fetal rat, the liver, lung, and kidney factor nickel group were lower than the sham operation group and the alloy group (P < 0.05), the postnatal 1D, and the 5D newborn rats were lower than the sham operation group and the alloy group (P < 0.05), 1 There was no significant difference in 0,15,20d neonatal rats. The local tissue formed inflammatory granuloma and necrosis at early 40d. The medium 80D was localized cystic tissue hyperplasia and hemorrhagic exudate, the type of tissue was rhabdomyedema, muscle fiber disorder, and coarse, the contents were broken red cells and a small number of lymphocytes, and the local mass increased in the late 160D. The pathological tissue type was embryonal rhabdomyosarcoma, and a large number of homogeneous deep dyed cancer cells were found. The contents were a large number of abnormal mesothelial cells and scattered cancer cells. There were no obvious structural abnormalities in the naked eyes of fetal rats. The retinal malformation in a nickel group was seen in a nickel group. The nickel group of the femoral head cartilage was less than the alloy group and the sham group, showing a decrease in the homologous cells in the cartilage proliferation area. The survival ability of the newborn rats was decreased, the number of death was increased, and the weight growth slowed down.

【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

【引证文献】