恒河猴肝移植模型急性排斥反应时T细胞亚群的变化（英文）

发布时间：2018-04-30 12:47

  本文选题：肝移植 + 恒河猴　； 参考：《中国组织工程研究》2014年49期

【摘要】：背景:寻找肝移植急性排斥反应的早期诊断指标,可快速而有效的评估移植后排斥反应的风险,而T淋巴细胞在急性排斥反应中发挥主要作用。目的:观察恒河猴肝移植后血液T淋巴细胞亚群的变化与急性排斥反应的关系。方法:采用改良血管袖套+胆道支撑管+动脉吻合法建立16例稳定的恒河猴肝移植模型,并将模型随机分为2组:实验组围手术期不给予免疫抑制治疗,对照组围手术期给予免疫抑制治疗,分别在移植后6,12,24,72 h共4个时间点收集血标本和肝组织,全自动生化分析仪测定丙氨酸氨基转移酶、天冬氨酸氨基转移酶、总胆红素,用流式细胞仪检测血CD4+/CD8+水平,然后取移植后肝脏组织进行苏木精-伊红染色以观察肝脏组织形态结构的改变,并根据Banff评分系统判断排斥反应程度。结果与结论:实验组恒河猴肝移植后12,24,72 h均有急性排斥反应发生,其中实验组丙氨酸氨基转移酶、天冬氨酸氨基转移酶和总胆红素在移植后24,72 h表达水平明显高于对照组(P0.05),实验组和对照组CD4+/CD8+的表达在移植后6 h即开始增高,实验组增高最明显,实验组移植后24,72 h CD4+/CD8+的表达明显高于对照组(P0.05),实验组移植后72 h肝脏组织形态病理改变程度重于对照组,Banff评分高于对照组(P0.05)。说明在肝移植后急性排斥反应早期,CD4+/CD8+的表达变化早于肝组织病理学及肝功能的改变。肝移植后血CD4+/CD8+水平的升高提示人体细胞免疫功能增强,对肝移植后急性排斥反应的早期诊断具重要意义。
[Abstract]:Background: looking for early diagnostic indicators of acute rejection in liver transplantation can quickly and effectively assess the risk of rejection after transplantation, and T lymphocytes play a major role in acute rejection. Aim: to observe the relationship between the changes of blood T lymphocyte subsets and acute rejection in rhesus monkey after liver transplantation. Methods: 16 stable rhesus monkey liver transplantation models were established by modified vascular cuff with bile duct arterial anastomosis. The models were randomly divided into two groups: the experimental group was not treated with immunosuppressive therapy during perioperative period. The control group was treated with immunosuppressive therapy during perioperative period. Blood samples and liver tissues were collected at 6: 12 ~ 2472 h after transplantation. The levels of alanine aminotransferase, aspartate aminotransferase and total bilirubin were measured by automatic biochemical analyzer. The level of CD4 / CD8 was detected by flow cytometry, and then the liver tissue was stained with hematoxylin and eosin to observe the changes of liver morphologic structure. The degree of rejection was judged according to the Banff score system. Results and conclusion: acute rejection occurred in the experimental group after liver transplantation in rhesus monkeys, and alanine aminotransferase was found in the experimental group. The expression of aspartate aminotransferase and total bilirubin was significantly higher than that of control group at 24 ~ 72 h after transplantation. The expression of CD4 / CD8 in experimental group and control group began to increase 6 hours after transplantation. The expression of CD4 / CD8 in the experimental group was significantly higher than that in the control group at 24 ~ 72 h after transplantation, and the degree of histopathological changes of liver in the experimental group was more serious than that in the control group at 72 h after transplantation. The results showed that the expression of CD4 / CD8 was earlier than that of liver histopathology and liver function in the early stage of acute rejection after liver transplantation. The increase of serum CD4 / CD8 level after liver transplantation indicates that the enhancement of human cellular immune function is of great significance for the early diagnosis of acute rejection after liver transplantation.
【作者单位】： 昆明医科大学附属甘美医院肝胆胰外科 云南省器官移植研究所肝移植研究中心;
【基金】：the Major Program of Kunming Science and Technology Bureau,No.08S100304~~
【分类号】：R657.3;R-332
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本文编号：1824614