共培养体系中小鼠淋巴细胞RNA编辑酶ADAR1的表达及ADAR1在小鼠细胞免疫应答中的作用研究

发布时间：2018-04-30 16:06

本文选题：共培养 + 半定量PCR　；参考：《第四军医大学》2006年硕士论文

【摘要】：RNA编辑是近年来发现的一种遗传信息修饰机制，它通过对前RNA特异位点的修饰，使腺嘌呤核苷转化为次黄嘌呤核苷(Adenosine-to-inosine，A-to-Ⅰ)，从而在转录后水平对目的基因进行修饰。接受编辑后的RNA翻译产物可能会比基因组编码的基因产物具有多样性，除了传统的蛋白翻译途径外，RNA编辑可以产生蛋白的同功异构体，从而在蛋白质多样性的形成中发挥重要的作用。越来越多的研究发现，在哺乳动物体内，许多与维持生命活动有关的重要功能蛋白质都受到A-to-ⅠRNA编辑的调控，使其结构和功能以及信号传导通路都受到影响。目前发现A-to-ⅠRNA编辑酶的底物多局限于中枢系统，而对Ⅰ-mRNA含量丰富的众多外周组织中的A-to-ⅠRNA编辑情况的研究相对较薄弱。在哺乳类已经克隆到四种RNA编辑酶(ADARs)，即ADAR1、ADAR2、ADAR3、ADATs，它们具有相似的催化结构域。本实验通过建立共培养细胞体系，观察小鼠淋巴细胞在异种抗原细胞的刺激下ADAR1的表达情况；并通过siRNA质粒下调小鼠淋巴细胞ADAR1的表达，，观察ADAR1表达抑制后的淋巴细胞免疫功能的变化。本实验首次系统的对ADAR1在不
[Abstract]:RNA editor is a genetic modification mechanism that has been discovered in recent years. It modifies the adenine nucleoside (Adenosine-to-inosine, A-to- I) by modifying the special site of the former RNA, and modifies the target gene at the post transcriptional level. The edited RNA translation product may be more than the genome encoded gene. In addition to the traditional protein translation pathway, RNA editors can produce isoisomers of protein and play an important role in the formation of protein diversity. More and more studies have found that in mammals, many important functional proteins related to the maintenance of life activities are edited by A-to- I RNA. The structure, function and signal transduction pathway are affected. The substrate of the A-to- I RNA editing enzyme is found to be limited to the central system, and the study of A-to- I RNA in the many peripheral tissues with rich I -mRNA content is relatively weak. In mammalian, four RNA editing enzymes (ADARs), namely ADAR1, have been cloned. ADAR2, ADAR3, ADATs, they have a similar catalytic domain. In this experiment, a co culture cell system was established to observe the expression of ADAR1 in mouse lymphocytes under the stimulation of xenogeneic antigen cells, and the expression of ADAR1 in mouse lymphocytes was down regulated by siRNA plasmid, and the changes of lymphocyte immune function after the inhibition of ADAR1 expression were observed. The first system of this experiment on ADAR1 is not

【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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