Erbin蛋白在GTS-21激活胆碱能抗炎通路中的调控作用

发布时间：2018-05-01 14:18

本文选题：胆碱能抗炎通路 + Erbin　；参考：《武汉大学学报(医学版)》2014年04期

【摘要】：目的:探讨Erbin蛋白在GTS-21激活胆碱能抗炎通路抑制胞壁酰二肽(MDP)诱导的炎症反应中的调控作用。方法:将正常培养的RAW264.7细胞分为4组:空白对照组(NS组),MDP组(M组):MDP(10μg/ml),GTS-21组(G组):MDP(10μg/ml)+α7nAChRs特异激动剂/GTS-21(50μg/ml),Erbin shRNA干扰组(R组):MDP(10μg/ml)+GTS-21(50μg/ml)+Erbin shRNA。在MDP刺激后1,6,24h时间点提取标本,每个时间点10个样本。Western检测Erbin的表达,免疫荧光检测Erbin的免疫定位,凝胶迁移率实验(EMSA印迹)检测NF-κB活化,ELISA检测TNF-α、IFN-γ等促炎性细胞因子水平。结果:MDP刺激后,Erbin表达升高,NF-κB活性增强,TNF-α、IFN-γ水平显著增加;与M组相比,G组Erbin表达明显升高(P0.05),NF-κB活性降低(P0.05),TNF-α、IFN-γ水平均下降(P0.05),与G组相比,R组Erbin表达明显下降(P0.05),NF-κB活性增强(P0.05),TNF-α、IFN-γ水平均升高(P0.05)。结论:Erbin蛋白是激活胆碱能抗炎通路抑制MDP诱导的炎症反应中关键调控蛋白,起负调控作用。
[Abstract]:Aim: to investigate the role of Erbin protein in the inhibition of inflammatory response induced by cholinergic anti-inflammatory pathway activated by GTS-21. Methods: the normal cultured RAW264.7 cells were divided into four groups: control group (NS group), MDP group (n = 10 渭 g / ml) and group G (n = 10 渭 g / ml) 伪 7nAChRs specific agonist, -GTS-21 (50 渭 g / ml), interfering group R (n = 10 渭 g / ml) GTS-21(50) Erbin shRNA (n = 10 渭 g / ml), the control group (n = 10 渭 g / ml), the G group (n = 10 渭 g / ml) and the G group (n = 10 渭 g / ml) a specific agonist for 伪 7nAChRs. Ten samples were collected at 24 h after MDP stimulation. The expression of Erbin was detected in 10 samples at each time point. The immunoreactivity of Erbin was detected by immunofluorescence, and the level of pro-inflammatory cytokines such as TNF- 伪 -IFN- 纬 was detected by Elisa. Results the expression of Erbin increased and the activity of NF- 魏 B increased the activity of TNF- 伪-魏 B increased significantly, the expression of Erbin increased significantly in group G and the activity of P0.05- 伪 -IFN- 魏 B decreased in group G. Compared with group G, the activity of Erbin increased significantly in group B and the activity of TNF- 伪 -IFN- 纬 increased in group G. Compared with group G, the activity of NF-kappa B in group B was significantly increased and the activity of TNF- 伪 -IFN- 纬 was increased. ConclusionErbin protein is a key regulatory protein that activates cholinergic anti-inflammatory pathway and plays a negative role in inhibiting inflammatory response induced by MDP.
【作者单位】：武汉大学中南医院麻醉科;
【基金】：国家自然科学基金资助项目(编号:81101449) 中央高校基本科研业务费专项资金(编号:111058) 湖北省自然科学基金资助项目(编号:2010CDB00404)
【分类号】：R392

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