G1型轮状病毒分子生物学检测及临床应用价值

发布时间：2018-05-02 04:41

本文选题：轮状病毒 + 轮状病毒G亚型　；参考：《中华实用诊断与治疗杂志》2014年12期

【摘要】：目的探讨G1型轮状病毒(rotavirus,RV)的分子生物学检测及其在RV感染性腹泻诊治中的应用价值。方法收集90例腹泻患儿第1、3天大便标本各90份,根据RV基因序列,结合文献设计针对RV G1型引物和探针,采用PCR法检测第1天标本中RV核酸,以免疫胶体金法检测第1、3天标本中RV抗体,比较2种方法检测阳性率。将PCR检测阳性病例随机分为2组,观察组立即行抗病毒及对症治疗,对照组在免疫胶体金法RV抗体阳性时再行上述治疗,比较2组住院时间。结果 PCR法共检出RV核酸51例,检测阳性率(56.7%)与第1天免疫胶体金法检测阳性率(6.7%)比较差异有统计学意义(P0.01),与第3天阳性率(48.9%,)比较差异无统计学意义(P0.05);观察组住院时间((8.0±2.5)d)低于对照组((15.0±1.5)d)(P0.05)。结论 PCR技术对RV G1型检测具有较高特异性和敏感性,有助于疾病的早期诊断与治疗。
[Abstract]:Objective to investigate the molecular biological detection of rotavirus type G 1 (RV) and its value in diagnosis and treatment of RV infectious diarrhea. Methods 90 stool specimens of 90 children with diarrhea on day 1 were collected. According to the sequence of RV gene and the design of literature, RV nucleic acid was detected by PCR method. The RV antibody was detected by immuno-colloidal gold assay in 3 days of the first day, and the positive rate of the two methods was compared. PCR positive cases were randomly divided into two groups. The observation group was treated with anti-virus and symptomatic therapy immediately, and the control group was treated again when the immuno-colloidal gold method RV antibody was positive. The hospitalization time of the two groups was compared. Results 51 cases of RV nucleic acid were detected by PCR. The positive rate (56.7%) was significantly higher than that on the first day (P 0.01g), but there was no significant difference compared with the third day (48.9%). The hospitalization time of the observation group was 8.0 卤2.5 days, which was lower than that of the control group (15.0 卤1.5 d). Conclusion PCR technique has high specificity and sensitivity for detection of RV G 1 type, which is helpful for early diagnosis and treatment of disease.
【作者单位】：郑州大学人民医院;河南省人民医院儿科;河南省人民医院检验科;
【分类号】：R373

【参考文献】