氟对小鼠生精细胞凋亡的影响及锌的保护作用

发布时间：2018-05-03 12:56

本文选题：氟化钠 + 锌　；参考：《广西大学》2007年硕士论文

【摘要】： 本研究通过对雄性小鼠腹腔注射氟化钠及醋酸锌建立氟中毒动物模型和治疗模型，采用石蜡切片、H-E染色、TUNEL末端原位标记及琼脂糖凝胶电泳的方法检测小鼠睾丸生精细胞的凋亡情况。旨在研究氟对成年小鼠生精细胞凋亡的影响及锌的保护作用，从而对基础理论研究及开发低毒鼠药以控制鼠害提供实验依据，并对经常接触氟的人群的生殖保健起到一定的参考指导作用。实验结果如下：小鼠腹腔注射氟化钠(20mgkg~(1-))后，睾丸生精细胞的显微结构出现了典型的细胞凋亡的形态特点。 TUNEL法显示，小鼠睾丸中凋亡细胞呈阳性反应，非凋亡细胞呈阴性反应。无论是对照组还是氟感染组和锌保护组，睾丸中凋亡的生精细胞多发生在精母细胞和精原细胞，而且是精母细胞凋亡最多；很少发生在精子细胞和精子。从细胞凋亡率可见，染氟组睾丸生精细胞细胞凋亡率显著高于对照组；染氟组的生精细胞凋亡率在腹腔注射第28天达到最大(11.972％)，以后逐渐回落。低剂量锌组(15mgkg~(-1))和高剂量锌组(30mgkg~(1-))的生精细胞凋亡率显著低于染氟组，并且高剂量锌组的凋亡指数与对照组差异不显著(P＞0.05)。琼脂糖凝胶电泳检测显示，对照组及锌保护组细胞DNA条带呈单一条带。染氟组凋亡细胞的DNA呈现特征性的“阶梯状”条带。可见，，氟化钠可以在体内诱导小鼠睾丸生精细胞凋亡；锌可以对这种凋亡起到保护作用。
[Abstract]:In this study, the animal model and treatment model were established by intraperitoneal injection of sodium fluoride and zinc acetate in male mice. The apoptosis of spermatogenic cells in testis of mice was detected by paraffin section, H-E staining, TUNEL terminal in situ labeling and agarose gel electrophoresis, and the effect of fluorine on the apoptosis of spermatogenic cells in adult mice was studied. The protective effect of zinc can provide experimental basis for basic theory research and development of low toxic rodent drugs to control rodent damage, and play a certain reference role to reproductive health care for people who often contact fluorine.
The results were as follows: after injection of sodium fluoride (20mgkg~ (1-)), the morphology of spermatogenic cells in the testis showed typical morphological characteristics of apoptosis.
TUNEL showed that the apoptotic cells in the testis of mice were positive, and the non apoptotic cells were negative.
The apoptotic spermatogonial cells in the testis were mostly in spermatocytes and spermatogonial cells in the control group or in the fluorine infection group and the zinc protective group, and the apoptosis was the most in spermatocytes, and the spermatocytes and spermatozoa were rarely occurring.
The apoptosis rate of testicular spermatogenic cells in the fluoride group was significantly higher than that in the control group. The apoptosis rate of spermatogenic cells in the fluorine group reached the maximum (11.972%) after twenty-eighth days of intraperitoneal injection (15mgkg~ (-1)) and the high dose zinc group (30mgkg~ (1-)) was significantly lower than that of the fluorine dyed group. There was no significant difference in the apoptotic index between the high-dose zinc group and the control group (P > 0.05).
The agarose gel electrophoresis showed that the DNA bands in the control group and the zinc protective group showed a single band. The DNA of the apoptotic cells in the fluorine stained group showed a characteristic "ladder like" strip.
It can be seen that sodium fluoride can induce apoptosis of spermatogenic cells in mice, and zinc can play a protective role in this apoptosis.

【学位授予单位】：广西大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

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