巴马长寿老人代谢相关基因GSTs、P450及抑癌基因P53的多态性研究
发布时间：2018-05-03 18:03
本文选题：GSTT1 + GSTM1 ； 参考：《广西医科大学》2006年硕士论文
【摘要】： 目的:探讨代谢相关基因GSTT1、GSTM1、CYP1A1及抑癌基因p53第4外显子第72位密码子(codon72)与人类长寿的关系。方法:应用PCR-RFLP技术对巴马156例(年龄90-105岁,平均93.2岁,长寿组)健康壮族长寿老人和142例健康壮族成年人(年龄20-72岁,平均34.8岁,对照组)的GSTT1、GSTM1、CYP1A1及p53基因进行基因分型,了解这些基因的多态性,分析各种基因(型)频率及多态性组合在长寿组和对照组之间的差异及其与长寿的关系。结果:长寿组的GSTT1基因、GSTM1基因的缺失(或携带)频率、CYP1A1基因的野生型(T/T)、纯合突变型(T/C)、杂合突变型(C/C)频率与对照组差别均无显著意义(p0.05);长寿组p53codon72片段的野生型(G/G)频率明显高于对照组(53%:29%,p0.05)。各种基因型的组合分析显示,对照组同时合并GSTT1、GSTM1、CYP1A1及p53codon72基因缺失或者突变的几率明显比长寿组为高,尤其是长寿组男性和女性的GSTT1(+)-GSTM1(-)-CYP1A1 (T/C,C/C)- p53coden72(G/C,C/C)基因组合及长寿组女性GSTT1(-)-GSTM1(-)-CYP1A1 (T/C,C/C)-p53coden72 (G/C,C/C)基因组合明显低于对照组( p0.05 );而长寿组男性GSTT1(-)-GSTM1(-)-CYP1A1 (T/C,C/C)-p53coden72 (G/G)基因组合高于对照组(p0.05),其它基因(型)组合在两组中无差别。结论:GSTT1、GSTM1、CYP1A1及p53基因的多态性与人类长寿有着较为密切的联系,其具体作用机制有待进一步研究。
[Abstract]:Aim: to investigate the relationship between the metabolism-related gene GSTT1GSTM1CY CYP1A1 and codon 72of p53 exon 4. Methods: genotyping of CYP1A1 and p53 genes in 156 healthy adults (aged 20-72 years, mean 34.8 years, control group) from 156 healthy adults (90-105 years old, average 93.2 years old) and 142 healthy adults (20-72 years old, mean 34.8 years old, control group) was performed using PCR-RFLP technique. To understand the polymorphism of these genes, and to analyze the differences between longevity group and control group and their relationship with longevity. Results: there was no significant difference in the frequency of deletion (or carrier) of GSTT1 gene, wild type of CYP1A1 gene, homozygous mutant T / C, heterozygous mutation type C / C between the longevity group and the control group (p 0.05), while the wild type G / G) frequency of the p53codon72 fragment in the longevity group was not significantly different from that in the control group. The rate was significantly higher than that of the control group. Combination analysis of different genotypes showed that the rate of deletion or mutation of CYP1A1 and p53codon72 gene in the control group was significantly higher than that in the longevity group. 灏ゅ叾鏄暱瀵跨粍鐢锋，
本文编号：1839522
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