窒息大鼠心肺复苏模型的建立及复苏早期脑水肿的相关研究

发布时间：2018-05-05 04:16

本文选题：窒息法 + 心肺复苏模型　；参考：《苏州大学》2006年硕士论文

【摘要】：第一部分窒息大鼠心肺复苏模型的建立目的：建立窒息大鼠心肺复苏模型；探讨心脏骤停(Cardiac arrest，CA)持续时间对复苏效果的影响。方法：雄性SD大鼠20只，随机分为2组：CA1min组；CA3min组。通过静脉注射肌松剂司可林模拟窒息的方法，使大鼠心脏骤停。心脏停跳持续1min或3min后实施心肺复苏，应用心电监护仪监测两组大鼠心肺复苏期间MAP、HR及体温的动态变化，股动脉采血监测血气的动态变化，并观察两组大鼠自主循环恢复时间、复苏成功率及复苏后3小时的存活情况。结果：1．CA1min和CA3min两组大鼠心肺复苏期间各项基本生命指征的变化趋势基本相同；但开始复苏之前，CA3min组大鼠的MAP和HR均明显低于CA1min组的大鼠(P＜0.05)，接近于0；两组大鼠ROSC时均存在较严重的代谢性酸中毒，提示此时补碱的必要性。 2．CA3min组大鼠的ROSC时间较CA1min组明显增加(P＜0.05)，，其复苏后3小时存活率较CA1min组明显降低(P＜0.05)。CA3min组的ROSC率与CA1min组比较，虽无显著性差异，但呈下降趋势。结论：1．成功建立窒息大鼠心肺复苏模型，模型基本稳定。 2．CA的持续时间及ROSC时间是决定心肺复苏成败及复苏预后的重要因素。CA时间及ROSC时间越长，复苏后长期存活率越低。第二部分心肺复苏早期大鼠脑组织含水量和水通道蛋白-4的动态变化及亚低温对其影响的研究目的：探讨复苏后早期脑水肿的动态变化，AQP4在其发生、发展中的作用，以及亚低温干预对二者的影响。方法：雄性SD大鼠48只，随机分为4组，分别为正常对照组、假手术组、常温复苏组、亚低温组，后两组各自进一步分为3个亚组：自主循环恢复后1、3和6小时组。建立窒息大鼠心肺复苏模型，应用干湿重法测定自主循环恢复后1、
[Abstract]:Part I Establishment of cardiopulmonary resuscitation model in asphyxia rats Aim: to establish cardiopulmonary resuscitation (CPR) model in asphyxiated rats and to investigate the effect of cardiac arrest CAA on resuscitation. Methods: twenty male Sprague-Dawley rats were randomly divided into 2 groups. Cardiac arrest was induced by intravenous injection of muscle relaxant Secolin to simulate asphyxia in rats. Cardiopulmonary resuscitation (CPR) was performed after cardiopulmonary resuscitation (1min) or cardiopulmonary resuscitation (3min). The dynamic changes of MAPHR and body temperature during cardiopulmonary resuscitation (CPR) were monitored in the two groups. The success rate of resuscitation and survival rate of 3 hours after resuscitation. Results 1. The changes of basic life indications in CA1min group and CA3min group were basically the same during cardiopulmonary resuscitation (CPR). Before resuscitation, the MAP and HR of rats in CA3min group were significantly lower than those in CA1min group (P < 0.05), and there were severe metabolic acidosis at ROSC in both groups, indicating the necessity of alkali supplementation. The ROSC time in 2.CA3min group was significantly higher than that in CA1min group (P < 0.05). The survival rate at 3 hours after resuscitation was significantly lower in 2.CA3min group than in CA1min group, and the ROSC rate in P < 0.05).CA3min group was significantly lower than that in CA1min group. Conclusion 1. The model of cardiopulmonary resuscitation in asphyxiated rats was established successfully, and the model was basically stable. The duration of 2.CA and the duration of ROSC were important factors to determine the success or failure of CPR and prognosis of CPR. The longer the duration of CA and ROSC, the lower the long-term survival rate after resuscitation. The second part: the dynamic changes of water content and aquaporin-4 in brain tissue of rats at the early stage of cardiopulmonary resuscitation and the effect of mild hypothermia on them. Aim: to investigate the dynamic changes of brain edema in the early stage after resuscitation and the role of AQP4 in the pathogenesis and development of brain edema and the effect of mild hypothermia intervention on them. Methods: Forty-eight male SD rats were randomly divided into 4 groups: normal control group, sham operation group, normothermic resuscitation group and mild hypothermia group. The cardiopulmonary resuscitation model of asphyxia rats was established.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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