抗结肠肿瘤相关抗原单克隆抗体的制备及鉴定

发布时间：2018-05-05 17:03

本文选题：肿瘤相关抗原 + 单克隆抗体　；参考：《细胞与分子免疫学杂志》2005年03期

【摘要】：目的:制备抗结肠肿瘤相关抗原的单克隆抗体(mAb)并对其进行初步的鉴定及功能研究。方法:将结肠癌细胞系或磨碎的结肠癌组织免疫小鼠,按常规方法进行细胞融合,采用活细胞周围花环形成的方法进行阳性克隆的筛选。用间接免疫荧光染色和流式细胞术分析及酶免疫组织化学的方法进行初步鉴定,并用抗体介导的补体依赖的细胞毒试验(CDC)进行初步的功能研究。结果:获得了2株分泌抗结肠肿瘤相关抗原mAb杂交瘤细胞株3A12和6A8。流式细胞仪检测,2株杂交瘤分泌的抗体均能在结肠癌细胞系中均为阳性。普通石蜡切片及组织芯片的免疫酶组织化学染色结果,显示2株mAb均在结肠肿瘤组织中有较高的阳性率。2株mAb分别与靶细胞及补体
[Abstract]:Objective: to prepare a monoclonal antibody against colonic tumor related antigen (mAb) and to make a preliminary identification and functional study. Method: immunize mice with colon cancer cell lines or grated colon cancer tissue to perform cell fusion according to conventional methods. The indirect immunofluorescence was used to screen the positive clones by using the ring of living cells. Preliminary identification of light staining and flow cytometry and enzyme immunohistochemistry, and preliminary functional studies using antibody mediated complement dependent cytotoxicity test (CDC). Results: 2 hybridoma cell lines secreted against colon tumor related antigen mAb, 3A12 and 6A8. flow cytometry, were detected, and 2 hybridoma secreted. The antibodies were all positive in the colon cancer cell lines. The results of immunohistochemical staining of common paraffin slices and tissue chips showed that 2 mAb strains had a high positive rate of.2 strain mAb in the colon tumor tissues and the target cells and complement respectively.

【作者单位】：第四军医大学基础部免疫学教研室第四军医大学基础部免疫学教研室第四军医大学基础部免疫学教研室第四军医大学基础部免疫学教研室第四军医大学基础部免疫学教研室第四军医大学基础部免疫学教研室第四军医大学基础部免疫学教研室
【基金】：ColoncancercollaborativeprogramwithCancerResearchInsti tute,NewYork,USA(国际合作项目,2002～2005年度)
【分类号】：R735.35

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