人粘膜病原微生物的分子致病机理及分子诊断学研究

发布时间：2018-05-06 03:12

本文选题：B族链球菌 + 脆弱类杆菌　；参考：《第一军医大学》2005年博士论文

【摘要】：包括共生菌在内的微生物遍布于人体的体表(如皮肤)或粘附于粘膜上皮组织(如胃肠道、呼吸道、阴道等处)。它们的总数远远超过人体细胞总数。在与其宿主长期共同演化的过程中,这些微生物发展了种类繁多的共生或致病因子和手段,以便于它们与宿主相互作用并引起一系列生物学反应,从而使得它们更好地侵入宿主细胞,并在其中长期生存,繁殖,甚至在某些情况下还引起宿主细胞癌变。因而,鉴定这些共生于人类的病原微生物的致病因子并研究其分子致病机理,以及对病原体进行早期诊断是有效预防和治疗其感染的关键所在。本研究综合应用细菌遗传学、细胞生物学、生物化学与分子生物学以及免疫学等多学科的研究方法,首先对两个重要的人体共生病原微生物B族链球菌(group B Streptococcus,GBS)和脆弱类杆菌(B.fragilis)的分子致病机理进行了研究;然后以重要致癌病原体人乳头瘤病毒(human papillomavirus,HPV)为对象,探索了限制性显示(restriction display,RD)和DNA芯片技术在病原体分子诊断中的应用前景。 B族链球菌(GBS)是一种革兰式阳性链球菌。它共生于多数人类胃肠道,并且也是引起新生儿脓血症,脑膜炎和肺炎等致命性感染的主要病原体。GBS的荚膜多糖(capsular polysaccahride,CPS)不仅是其主要的致病决定因子,而
[Abstract]:Microbes, including symbiotic bacteria, are found on the body's surface (e.g. skin) or on mucosal epithelium (e.g., gastrointestinal tract, respiratory tract, vagina, etc.). Their total number far exceeds the total number of human cells. In the course of long-term co-evolution with their host, these microbes have developed a wide variety of symbiotic or pathogenic factors and means to facilitate their interaction with the host and to trigger a series of biological responses, It makes them invade host cells better and survive, reproduce and even cause carcinogenesis of host cells in some cases. Therefore, the key to effectively prevent and treat the infection is to identify the pathogenetic factors of these pathogenic microorganisms, to study their molecular pathogenic mechanism, and to make early diagnosis of pathogens. In this study, a comprehensive application of bacterial genetics, cell biology, biochemistry and molecular biology, as well as immunology and other multidisciplinary research methods, The molecular pathogenesis of two important human symbiotic pathogens, group B Streptococcus sp. GBSand B. fragilis, was studied, and then the human papillomavirus HPVs, an important carcinogenic pathogen, were studied. The application prospect of restrictive display restriction (RDX) and DNA chip technology in pathogen molecular diagnosis was explored. Group B streptococcus (GBS) is a Gram-positive streptococcus. It is symbiotic in the gastrointestinal tract of most humans and is also a major pathogen of neonatal sepsis, meningitis and pneumonia. The capsule polysaccharide encapsular capsular polysaccahridea (CPS) is not only its main pathogenic determinant, but also a major pathogen of neonatal sepsis, meningitis and pneumonia.
【学位授予单位】：第一军医大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R37

【参考文献】