白念珠菌耐药基因CDR1、CDR2、MDR1表达与氟康唑耐药的相关性分析

发布时间：2018-05-07 19:33

本文选题：念珠菌 + 白色　；参考：《重庆医学》2014年03期

【摘要】：目的探讨对氟康唑耐药白念珠菌分离的耐药基因CDR1、CDR2和MDR1在敏感株和耐药株中表达水平。方法从送检样本中分离并鉴定出白念珠菌,选取其中115株采用微量稀释法测定对氟康唑耐药的白念珠菌最小抑菌浓度(MIC)值,并采用PCR方法检测白念珠菌耐药基因在敏感株和耐药株中表达情况。结果 115株白念珠菌对氟康唑的半数抑菌范围为5μg/mL,90%抑菌范围为126μg/mL;其中76株敏感,39株耐药,敏感率为66.1%,耐药率为36.8%。CDR2基因△CT值敏感株组为9.33±3.21,耐药株组为7.49±2.54,两组比较差异有统计学意义(t=2.659,P0.05);CDR1基因和MDR1基因的表达水平在敏感株和耐药株中比较差异无统计学意义(P0.05)。结论白念珠菌耐药是个较为复杂的过程,其中白念珠菌对氟康唑耐药与CDR2高表达有关,与CDR1和MDR1表达无关。
[Abstract]:Objective to investigate the expression level of CDR1, CDR2 and MDR1 in the sensitive and resistant strains of fluconazole resistant Candida albicans. Methods Candida albicans were isolated from the samples and 115 of them were selected to determine the minimum inhibitory concentration (MIC) of Candida albicans resistant to fluconazole, and the PCR method was used. The expression of Candida albicans resistance gene in sensitive and drug-resistant strains was detected. Results 115 strains of Candida albicans were 5 micron g/mL for fluconazole and 126 micron g/mL, 90% of them were sensitive, 39 strains were resistant, the sensitivity was 66.1%, the resistance rate of 36.8%.CDR2 gene was 9.33 + 3.21, and the drug resistant group was 7.49 + 2. .54, the two groups were statistically significant (t=2.659, P0.05), and the expression level of CDR1 and MDR1 genes was not statistically significant in sensitive and drug-resistant strains (P0.05). Conclusion Candida albicans resistance is a more complex process, in which Candida albicans resistance to fluconazole is related to the high expression of CDR2 and is not related to the expression of CDR1 and MDR1.

【作者单位】：河北医科大学第四医院检验科;北京市结核病胸部肿瘤研究所中心实验室;中国医学科学院北京协和医学院皮肤病研究所真菌科;
【基金】：国家自然科学基金资助项目(81101203)
【分类号】：R346

【参考文献】