内皮细胞硫酸乙酰肝素蛋白聚糖与细胞粘附关系的实验研究

发布时间：2018-05-08 16:21

本文选题：内皮细胞 + 蛋白聚糖　；参考：《山西医科大学》2005年博士论文

【摘要】：目的细胞粘附是多细胞生物维持本身结构和功能极为重要的生物学现象,是细胞和细胞之间的一种信息交流方式,同各种病理、生理过程紧密相关。血管内皮细胞是休克、创伤、炎症、肿瘤时各种细胞粘附的靶细胞。细胞粘附机理十分复杂,多种活性物质参与其中。经典的粘附分子是决定细胞粘附过程的主要物质,而细胞膜表面的主要蛋白聚糖—硫酸乙酰肝素蛋白聚糖(heparin sulfate proteoglycans,HSPGs)由于其多样化的结构功能和在细胞识别、细胞粘附中的作用日益受到重视,成为当今研究的热点之一。本实验在体外培养人脐静脉内皮细胞并稳定传代后作为实验细胞;首先明确培养的人脐静脉内皮细胞是否能表达HSPGs家族两个重要成员——Syndecan-1和Glypican-1;以二者为研究指标,以多种外源性干预因素从不同途径作用于内皮细胞HSPGs;观察内皮细胞HSPGs在干预因素作用下的变化趋势,评价各种干预因素对内皮细胞HSPGs的影响;以分离的正常人外周血单个核细胞与内皮细胞的粘附为研究模型,观察内皮细胞HSPGs改变对细胞粘附变化的直接影响,探讨内皮细胞HSPGs在细胞粘附中的作用。以采集的急性白血病病人血清作用于体外培养的内皮细胞,模拟上白血病时内皮细胞在病理性血清作用下活化的情况,观察内皮细胞HSPGs在转录水平和蛋白水平的表达变化,以正常人外周血分离的单个核细胞与白血病血清活化后的内皮细胞的粘附变化为研究模型,探讨白血病情况下,内皮细胞HSPGs同细胞粘附的关系,为预防和治疗白血病髓外浸润提供新的思路和方法。
[Abstract]:Purpose Cell adhesion is a very important biological phenomenon in maintaining the structure and function of multicellular organisms. It is a way of information exchange between cells and cells. It is closely related to various pathological and physiological processes. Vascular endothelial cells are target cells that adhere to various cells during shock, trauma, inflammation, and tumour. The mechanism of cell adhesion is very complex, in which many active substances are involved. Classical adhesion molecules are the main substances that determine the cell adhesion process, while the main proteoglycan proteoglycans of heparin sulfate (heparin sulfate proteoglycansan HSPGs) on the surface of the cell membrane, due to their diverse structural functions and recognition in cells, More and more attention has been paid to the role of cell adhesion, which has become one of the hot research topics. In this study, human umbilical vein endothelial cells were cultured in vitro and subcultured stably as experimental cells. Firstly, whether the cultured human umbilical vein endothelial cells could express two important members of the HSPGs family, Syndecan-1 and Glypican-1, was determined. The changes of HSPGs in endothelial cells were observed and the effects of various intervention factors on HSPGs of endothelial cells were evaluated. The adhesion of human peripheral blood mononuclear cells (PBMC) to endothelial cells (ECs) was used as a model to observe the direct effect of endothelial cell HSPGs changes on cell adhesion and to explore the role of endothelial cell HSPGs in cell adhesion. In order to observe the expression of HSPGs at transcription level and protein level, the collected serum of acute leukemia patients acted on endothelial cells cultured in vitro, and simulated the activation of endothelial cells under the action of pathological serum during upper leukemia. The relationship between endothelial cell HSPGs and leukemic cell adhesion was studied by using normal human peripheral blood mononuclear cells (PBMC) and leukemic serum activated endothelial cells (LECs) as a model to investigate the relationship between endothelial cell adhesion (HSPGs) and leukemic cell adhesion. To provide new ideas and methods for the prevention and treatment of extramedullary infiltration of leukemia.
【学位授予单位】：山西医科大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R363

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