D-半乳糖诱导小鼠mtDNA损伤的研究

发布时间：2018-05-12 07:48

本文选题：衰老 + 动物模型　；参考：《第四军医大学》2005年硕士论文

【摘要】：衰老是不可抗拒的自然规律,其发生发展是一个难解的谜团。近年来,我国逐步进入老龄化社会,有效地维护老年人的健康,防治衰老相关性疾病成为人们普遍关注的社会问题。因此,研究衰老发生机制及延缓衰老进程,也成为当今学术界倍受重视的领域。关于衰老,人们从不同角度提出了多种学说,总的来说可以分为两大类:一种认为衰老是人体固有的、随时间退化的过程,称为自然演进学说;另一种是认为衰老是各种内外损伤积累的结果,称为环境伤害学说。衰老的过程是内外因素共同作用的结果,这两类学说各自强调了其中一方面因素。近年来兴起的衰老的线粒体-mtDNA学说认为,线粒体呼吸功能衰老和mtDNA结构损伤之间的恶性循环是导致机体衰老的重要原因。此学说既肯定了遗传物质对衰老进程的重要作用,又没有忽略环境损伤因素的影响,此学说一经提出就得到了广大学者的支持,成为当今衰老研究的一大热点。在衰老的研究中,合适的衰老动物模型对于深入研究衰老性疾病的发生机制、发展过程非常重要,也是抗衰老药物的筛选、研制所必需的前提。近年来,中外学者通过给大鼠或小鼠注射大剂量D-半乳糖制作了亚急性衰老动物模型,并将此模型运用到衰老研究中,特别是抗衰老中药的筛选研究。但是,学界对此动物模型的研究一直不够深入,对其发生机理的阐述不够明确。深入了解伴随动物造模发生的一系列生化及分子生物学的改变,对于合理使用此种模型动物具有重大意义。衰老的线粒体-mtDNA学说的提出,为深入研究D-半乳糖致衰老动物模型提供了新的理论支持。设计本课题,建立亚急性衰老动物模型。通过测定线粒体呼吸酶活性以观察受试动物线粒体功能的衰老情况;通过测定MDA含量以及抗氧化酶
[Abstract]:Aging is an irresistible law of nature, its occurrence and development is a mystery. In recent years, China has gradually entered an aging society, effectively maintaining the health of the elderly and preventing and curing age-related diseases has become a social issue of universal concern. Therefore, the research on the mechanism of aging and the process of delaying aging has become an important field in academic circles. On aging, people put forward a variety of theories from different angles, in general can be divided into two categories: one is that aging is inherent in the human body, the process of degradation with time, called natural evolution theory; The other is that aging is the result of a variety of internal and external damage accumulation, called environmental injury theory. The process of aging is the result of internal and external factors, and the two theories emphasize one of them. In recent years, the mtDNA theory of aging suggests that the vicious circle between mitochondrial respiratory function senescence and mtDNA structural damage is an important cause of aging. This theory not only affirms the important role of genetic material in aging process, but also does not ignore the influence of environmental damage factors. As soon as this theory is put forward, it has been supported by many scholars and has become a hot spot in the field of aging research. In the research of aging, the suitable animal model of aging is very important for the further study of the mechanism of aging diseases and the development process. It is also the prerequisite for the screening of anti-aging drugs and the development of anti-aging drugs. In recent years, Chinese and foreign scholars have made subacute aging animal model by injecting large dose of D-galactose into rats or mice, and applied this model to the research of aging, especially the screening of anti-aging Chinese medicine. However, the research on the animal model has not been deep enough, and the mechanism of its occurrence is not clear enough. A deep understanding of a series of biochemical and molecular biological changes associated with animal modeling is of great significance for the rational use of this model animal. The mtDNA theory of aging provides a new theoretical support for the further study of aging animal model induced by D-galactose. Design this subject, establish the animal model of subacute senescence. The aging of mitochondria function was observed by measuring the activity of mitochondrial respiratory enzyme, and the content of MDA and antioxidant enzymes were measured.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R-332

【引证文献】