Notch信号通路对VEGF促大鼠间充质干细胞增殖的作用

发布时间：2018-05-12 11:19

本文选题：间充质干细胞 + Notch信号通路　；参考：《中国实验血液学杂志》2014年04期

【摘要】：本研究旨在探讨Notch信号传导通路在VEGF促大鼠间充质干细胞增殖中的作用。体外培养大鼠间充质干细胞,取对数生长期的细胞用于实验研究。用Notch通路阻断剂DAPT阻断Notch信号传导,观察该通路在VEGF作用下大鼠间充质干细胞的增殖情况。实验分为4组:空白对照组、VEFG组、DAPT组及VEGF+DAPT组。应用CCK-8法检测各组细胞增殖情况,RT-PCR法检测各组相关基因(Notch1、Notch2、Flk-1、HES-1)mRNA水平的变化。结果表明,细胞培养3 d,各时间点(24 h,48 h,72 h)DAPT组及VEGF+DAPT组细胞存活率均较低,细胞形态变圆脱落,数目明显减少;VEGF组存活率最高,差异具有统计学意义(P0.01);与对照组相比,VEGF组的Notch1、Notch2和Flk-1的表达水平均上调,而DAPT组及VEGF+DAPT组Notch1和Notch2的表达水平均下调,差异均具有统计学意义(P0.05);VEGF组Hes-1基因水平下调,而DAPT组及VEGF+DAPT组Hes-1基因水平上调,差异具有统计学意义(P0.05);DAPT组及VEGF+DAPT组的Flk-1基因表达水平稍有下调,但差异无统计学意义(P0.05)。结论:Notch信号通路在VEGF作用下对大鼠间充质干细胞的增殖具有明显促进作用,而DAPT可抑制这种增殖效应。
[Abstract]:The aim of this study was to investigate the role of Notch signaling pathway in the proliferation of rat mesenchymal stem cells stimulated by VEGF. Rat mesenchymal stem cells were cultured in vitro. Notch signal transduction was blocked by DAPT, a Notch pathway blocker, and the proliferation of rat mesenchymal stem cells induced by VEGF was observed. The experiment was divided into 4 groups: blank control group (VEFG) and VEGF DAPT group. CCK-8 assay was used to detect cell proliferation in each group. RT-PCR was used to detect the mRNA level of Notch1ntch2Flk-1pHES-1mRNA. The results showed that the cell survival rate was lower, the cell morphology became round and shedded, and the number of cells decreased significantly at 24 h or 48 h ~ 72 h)DAPT, and the survival rate was the highest in the VEGF DAPT group at 3 days after cell culture. Compared with the control group, the expression of Notch1ntch2 and Flk-1 were up-regulated, while the expression of Notch1 and Notch2 were down-regulated in DAPT group and VEGF DAPT group. However, the level of Hes-1 gene was up-regulated in DAPT group and VEGF DAPT group, and the difference was statistically significant. The expression of Flk-1 gene in DAPT group and VEGF DAPT group was slightly down-regulated, but the difference was not statistically significant (P 0.05). Conclusion the VEGF can promote the proliferation of rat mesenchymal stem cells by VEGF, and DAPT can inhibit the proliferation of rat mesenchymal stem cells.
【作者单位】：广东医学院附属医院儿童医学中心;
【基金】：湛江市财政资金科技专项竞争性分配项目(2012C0302)
【分类号】：R329.2

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