甜味刺激与酒精嗜好对大鼠脑内c-Fos表达的影响及其相关性研究

发布时间：2018-05-12 15:21

本文选题：大鼠 + 酒精嗜好　；参考：《河北师范大学》2007年硕士论文

【摘要】： 味觉与酒精嗜好的关系已被人们广泛研究,已经知道,大鼠脑室内慢性给以酒精可以引起对酒精的嗜好性,并同时增加与摄食和报酬有关脑区内的c-fos表达。Blednov等通过对突变小鼠的研究指出,突变小鼠对酒精的摄取量与其对酒精的口腔感觉报酬有关,并且小鼠对酒精的口腔感觉报酬与甜味感觉有关。Lemon等也指出,在中枢神经系统内的味觉活动上,酒精与甜味具有相似的特征。虽然酒精与甜味溶液的摄取之间存在着很强的正相关,但是该相关的神经机制尚不清楚,近来的数据提示与味觉基础有关。那么甜味刺激在正常大鼠脑内所引起的c-fos表达与口腔酒精刺激酒精嗜好性大鼠在脑内所引起的c-fos表达有什么异同呢?酒精嗜好性大鼠戒断后与正常大鼠和酒精依赖大鼠在行为学上又有什么差异呢?为了了解这些问题,本研究设计了如下实验,对此进行初步探讨。实验:用两瓶法分别测定正常大鼠对酒精/水、蔗糖/水、0.2mol/L蔗糖/0.2mol/L蔗糖6%浓度酒精混合液的嗜好比。在上述实验的基础上建立大鼠的酒精嗜好模型,实验方法为酒精递增蔗糖递减法。观察在建立过程中,模型大鼠对酒精的嗜好性与正常大鼠酒精嗜好性之间的差异,然后给模型大鼠口腔酒精刺激,观察其与正常大鼠糖精刺激、酒精刺激在脑内相应核团c-fos表达的差异。所的结果和分析如下: 1、正常大鼠对甜味刺激具有天生的嗜好性,实验证明,在提供食物选择时,大鼠总是喜欢高脂肪或者高糖食物。包括人在内的几乎所有杂食性动物都有先天性的甜味嗜好性和苦味回避性。 2、正常大鼠对于酒精的嗜好性远远低于蔗糖的嗜好性,大鼠对于酒精是厌恶的。低浓度的酒精与蔗糖混合后却大大的增加了大鼠的味觉嗜好性。提示风味在酒精的嗜好性中起到了关键作用。 3、模型大鼠戒断后表现出,情绪烦躁、易怒、毛色发暗、粪便变软、体重减轻等异常。提示长期饮用酒精可能导致了脑内神经机制相应变化。 4、正常大鼠口腔酒精、糖精刺激与模型大鼠口腔酒精刺激在c-fos表达上的差异主要出现在腹侧上橄榄周区、杏仁内侧核、下丘脑室旁核、菱形核、丘脑室旁核、臂旁外侧核。提示大鼠的味觉嗜好性变化是脑内多个核团共同作用的结果。
[Abstract]:The relationship between taste and alcohol addiction has been widely studied. At the same time, we also increased the expression of c-fos. Blednov and so on in the brain area related to food intake and reward. Through the study of mutant mice, it was pointed out that the amount of alcohol intake in mutant mice was related to the oral sensory reward of alcohol. It was also pointed out that alcohol and sweet taste have similar characteristics in taste activity in central nervous system. Although there is a strong positive correlation between alcohol intake and sweet solution uptake, the related neural mechanism is unclear, and recent data suggest that it is related to the taste basis. So what are the similarities and differences between the c-fos expression induced by sweet taste stimulation in normal rat brain and the c-fos expression induced by oral alcohol stimulation in alcohol addicted rats? What is the difference in behavior between rats with alcohol addiction and normal and alcohol dependent rats after abstinence? In order to understand these problems, the following experiments are designed and discussed. Experiment: the ratio of alcohol / water, sucrose / water 0.2mol / L sucrose to 0.2mol / L sucrose 6% alcohol mixture in normal rats was determined by two bottles method. On the basis of the above experiments, a model of alcohol addiction in rats was established. During the course of establishment, the difference between the alcohol addiction of model rats and that of normal rats was observed, and then the oral alcohol stimulation was given to the model rats, and the saccharin stimulation was observed between the model rats and the normal rats. The difference of c-fos expression in the corresponding nuclei of the brain stimulated by alcohol. The results and analysis are as follows: 1. Normal rats have a natural preference for sweet taste stimulation. The experiment shows that the rats always prefer high-fat or high-sugar foods when providing food choices. Almost all omnivorous animals, including humans, have congenital sweetness and bitterness avoidance. 2. Alcohol addiction in normal rats is much lower than that in sucrose, and alcohol is abhorrent in rats. Low concentration of alcohol mixed with sucrose significantly increased the taste appetite of rats. It is suggested that flavor plays a key role in alcohol addiction. 3. After abstinence, the model rats showed irritability, dark hair color, soft feces, weight loss and so on. The results suggest that drinking alcohol for a long time may lead to the corresponding changes of neural mechanism in the brain. 4. The difference of c-fos expression between normal rats and model rats was mainly found in ventral superior olive area, medial amygdaloid nucleus, paraventricular nucleus of hypothalamus, rhombus nucleus, paraventricular nucleus of thalamus and lateral parabrachial nucleus. The results suggest that the changes of taste preference in rats are the result of the interaction of multiple nuclei in the brain.
【学位授予单位】：河北师范大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R33

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