胰岛素抵抗动脉粥样硬化小型猪模型的研究

发布时间：2018-05-12 18:18

本文选题：藏猪 + 胰岛素抵抗　；参考：《中国比较医学杂志》2014年01期

【摘要】：目的观察高脂诱导对藏猪糖脂代谢和粥样斑块形成的影响,探讨藏猪胰岛素抵抗动脉粥样硬化模型的病症特点。方法取4~5月龄藏猪随机分为正常对照组4只饲喂普通饲料、AS模型组共8只饲喂高脂饲料,连续造模24周。检测造模前、造模后4、8、12、16、20、24周的空腹血糖、果糖胺、血脂、血清胰岛素含量测定和糖耐量试验,并计算动脉硬化指数(AI)、胰岛素抵抗指数(HOMA-IR)和胰岛素敏感指数(HOMA-IS)。造模后24周,测定麻醉状态下动脉血压和左心室功能指标,并取主动脉进行大体观察和HE染色及油红"O"染色。结果与正常对照组比,AS模型组从第16周开始空腹血糖显著升高(P0.05,P0.01),果糖胺从第4周起即发生显著升高(P0.01)血脂水平亦在造模第4~24周时升高显著(P0.01),AS模型组藏猪在第4~24周期间AI显著上升(P0.05,P0.01),造模第16~24周HOMA-IR亦显著升高(P0.05,P0.01),HOMA-IS则显著降低(P0.05)。第24周时,AS模型组MBP和SBP显著升高(P0.05,P0.01),LVEDP、+dp/dt max、-dp/dt max较正常组均发生显著性改变(P0.05,P0.01),病理学观察显示模型组出现明显的脂质沉积和粥样硬化形成。结论藏猪高脂饲料诱导后能形成明显的动脉粥样硬化,并伴有高血压、高血脂、糖耐量异常等胰岛素抵抗症状,是研究的胰岛素抵抗动脉粥样硬化的理想的实验动物。
[Abstract]:Objective to observe the effects of hyperlipidemia on glucose and lipid metabolism and atherosclerotic plaque formation in Tibetan pigs and to explore the characteristics of insulin resistance atherosclerosis model in Tibetan pigs. Methods four five-month-old Tibetan pigs were randomly divided into normal control group (n = 4) and normal diet group (n = 8) fed with high fat diet for 24 weeks. Fasting blood glucose, fructosamine, blood lipid, serum insulin content and glucose tolerance test were measured at 24 weeks before and after modeling. The arteriosclerosis index (AAI), insulin resistance index (HOMA-IRI) and insulin sensitivity index (HOMA-ISN) were calculated. After 24 weeks, arterial blood pressure and left ventricular function were measured under anesthesia, and aorta were taken for general observation, HE staining and oil red "O" staining. Results compared with the normal control group, the fasting blood glucose in the as model group increased significantly from the 16th week onwards, and fructosamine increased significantly from the 4th week to the 4th week. During the period, AI increased significantly (P0.05) and P0.01 (P0.01), HOMA-IR also increased significantly in the 1624 week of modeling, while HOMA-IS decreased significantly. At the 24th week, the MBP and SBP of as model group were significantly higher than that of normal group. The changes of dp/dt max-dp / DT max and dp/dt max-dp / DT max were significantly higher than those of normal group. The pathological observation showed that lipid deposition and atherosclerosis appeared obviously in the model group. Conclusion High fat diet can induce significant atherosclerosis, accompanied by hypertension, hyperlipidemia, impaired glucose tolerance and other insulin resistance symptoms. It is an ideal experimental animal of insulin resistance atherosclerosis.
【作者单位】：浙江中医药大学动物实验研究中心/比较医学研究中心;
【基金】：浙江省自然科学基金(LY12C04002) 浙江省卫生高层次创新人才培养工程项目
【分类号】：R-332

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